Hallen-Adams Heather E, Cavinder Brad L, Trail Frances
Department of Plant Biology, Michigan State University, East Lansing, MI, USA.
Methods Mol Biol. 2011;722:79-101. doi: 10.1007/978-1-61779-040-9_6.
Fusarium graminearum, the causal agent of head blight of wheat, was the third filamentous fungus to have a completed genome sequence. Since the release of the genome sequence in 2003, F. graminearum has become a model for studies of genomics and transcriptomics, mycotoxins, fungal population genetics, gene function, and sexual development. Herein we present the techniques we have used in our laboratory to perform expression analyses on life cycle stages of F. graminearum and techniques to functionally characterize those genes identified as potentially interesting.
禾谷镰刀菌是小麦赤霉病的病原菌，是第三种完成基因组测序的丝状真菌。自2003年基因组序列发布以来，禾谷镰刀菌已成为基因组学和转录组学、霉菌毒素、真菌群体遗传学、基因功能和有性发育研究的模型。在此，我们介绍了我们实验室用于对禾谷镰刀菌生命周期阶段进行表达分析的技术，以及对那些被鉴定为可能有趣的基因进行功能表征的技术。
