Department of Plastic, Reconstructive, and Aesthetic Surgery, Edouard Herriot Hospital, 5 Place d'Arsonval, 69437, Lyon Cedex 03, France.
Aesthetic Plast Surg. 2011 Dec;35(6):1061-72. doi: 10.1007/s00266-011-9734-8. Epub 2011 May 18.
Soft tissue engineering offers new perspectives for improving fat graft survival, for which the appropriate association of cells and scaffold seems essential. This study aimed to analyze the survival of free-cell grafts compared with adipose-derived stem cells (ASCs) seeded on collagen scaffolds.
Adipose tissue from a single volunteer was used for the following preparations: purified adipose tissue, isolated mature adipocytes (free-cell graft), cultured ASCs without scaffold (free-cell graft), collagen scaffold only, cultured ASCs in collagen scaffold without and with bioactive factors, and freshly-isolated ASCs in collagen scaffold. These were grafted on 18 nude mice for 2 months, after which specimens were evaluated grossly and histologically using hematoxylin-phloxine-safran (HPS), Oil-Red-O, and antivimentin labeling. Specimens and animals were weighed before implantation and after explantation, and weight values were statistically analyzed.
Free-cell grafts (mature adipocytes and free ASCs) showed complete resorption in 50 and 60% of the animals (remaining weight fraction was 22.5 and 5.3%, respectively). The survival of purified adipose tissue was 81.8% (statistically greater compared with free-cell grafts; p < 0.05). In the ASCs-scaffold association, the remaining weight fractions (87.3-70.4%) were statistically greater than in free-cell grafts (5.3-22.5%; p < 0.05), but the difference between ASC-scaffolds and fat grafts was not statistically significant. These results were confirmed by clinical and histologic observations.
Three-dimensional collagen scaffolds seem to improve survival of ASCs compared with free-cell grafts (adipocytes and free ASCs).
软组织工程为提高脂肪移植物的存活率提供了新的视角，而细胞与支架的适当结合似乎至关重要。本研究旨在分析游离细胞移植物与脂肪来源干细胞（ASCs）接种在胶原支架上的存活率。
使用一位志愿者的脂肪组织进行以下制备：纯化的脂肪组织、分离的成熟脂肪细胞（游离细胞移植物）、无支架培养的 ASCs（游离细胞移植物）、仅胶原支架、无生物活性因子和有生物活性因子的胶原支架培养的 ASCs 以及新鲜分离的胶原支架中的 ASCs。将这些移植物移植到 18 只裸鼠身上 2 个月，然后对标本进行大体和组织学评估，使用苏木精-派洛宁-固绿（HPS）、油红-O 和抗波形蛋白标记。在植入前和取出后对标本和动物进行称重，并对重量值进行统计学分析。
游离细胞移植物（成熟脂肪细胞和游离 ASCs）在 50%和 60%的动物中完全吸收（剩余重量分数分别为 22.5%和 5.3%）。纯化脂肪组织的存活率为 81.8%（与游离细胞移植物相比具有统计学意义；p<0.05）。在 ASC-支架结合中，剩余的重量分数（87.3-70.4%）明显高于游离细胞移植物（5.3-22.5%；p<0.05），但 ASC-支架与脂肪移植物之间的差异无统计学意义。这些结果得到了临床和组织学观察的证实。
与游离细胞移植物（脂肪细胞和游离 ASCs）相比，三维胶原支架似乎可以提高 ASCs 的存活率。
