Department of Clinical and Biological Sciences, Oral Medicine and Oral Oncology Section, University of Turin, Italy.
Cancer. 2011 Nov 15;117(22):5052-7. doi: 10.1002/cncr.26126. Epub 2011 May 16.
Oral verrucous carcinomas (OVCs) are characterized by better prognosis than oral squamous cell carcinomas (OSCCs). Because chromosomal instability (CIN) in solid tumors is indicative of prognosis, this study investigated whether OVCs and OSCCs were characterized by differences in CIN biomarkers.
Fresh or frozen multiple tissue samples were submitted to high-resolution DNA flow cytometry (hr DNA-FCM).
DNA aneuploid sublines were detected in 6 of 9 OVCs (66.7%) and in 20 of 25 OSCCs (80.0%). Multiple DNA aneuploid sublines were observed, respectively, in 2 of 6 (33.3%) DNA aneuploid OVCs and in 14 of 20 (70%) DNA aneuploid OSCCs (P = .163). OVCs were mainly characterized by DNA Index (DI) values in the near-diploid region (DI≠1 and DI < 1.4), whereas aneuploid OSCCs carried most frequently multiple aneuploid sublines with high DI values (DI ≥ 1.4). DNA near-diploid and high aneuploid sublines were, respectively, 87.5% and 12.5% for the OVCs versus 30% and 70% for the OSCCs (P = .004).
Present data suggest that OVCs are characterized by a lower degree of CIN and tumor heterogeneity than OSCCs, such that they appear as "frozen" in an early stage of DNA near-diploid aneuploidy, as previously observed for oral preneoplastic lesions. These DI characteristics, which can easily be obtained by hr DNA-FCM, appear to reflect the well-known differences in aggressiveness and prognosis of OVCs and OSCCs.
口腔疣状癌（OVC）的预后优于口腔鳞状细胞癌（OSCC）。由于实体瘤中的染色体不稳定性（CIN）提示预后，因此本研究调查了 OVC 和 OSCC 是否具有不同的 CIN 生物标志物特征。
提交新鲜或冷冻的多个组织样本进行高分辨率 DNA 流式细胞术（hr DNA-FCM）检测。
在 9 例 OVC 中有 6 例（66.7%）和 25 例 OSCC 中有 20 例（80.0%）检测到 DNA 非整倍体细胞系。分别在 6 例 DNA 非整倍体 OVC 中有 2 例（33.3%）和在 20 例 DNA 非整倍体 OSCC 中有 14 例（70.0%）观察到多个 DNA 非整倍体细胞系（P =.163）。OVC 主要以接近二倍体区域的 DNA 指数（DI）值为特征（DI≠1 且 DI < 1.4），而非整倍体 OSCC 则携带高频带多个具有高 DI 值（DI ≥ 1.4）的非整倍体亚系。接近二倍体 DNA 亚系和高非整倍体亚系分别为 87.5%和 12.5%，OSCC 为 30%和 70%（P =.004）。
目前的数据表明，OVC 的 CIN 程度和肿瘤异质性低于 OSCC，表现为在早期的 DNA 接近二倍体非整倍体阶段就已经“冻结”，这与先前观察到的口腔癌前病变相似。这些可通过高分辨率 DNA-FCM 轻松获得的 DI 特征似乎反映了 OVC 和 OSCC 之间众所周知的侵袭性和预后差异。
