一种用于检测 [PSI⁺] 朊病毒的遗传背景下 [URE3] 朊病毒的新型显色测定法。
A new colour assay for [URE3] prion in a genetic background used to score for the [PSI⁺] prion.
机构信息
Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, Chicago, USA.
出版信息
Yeast. 2011 Jul;28(7):555-60. doi: 10.1002/yea.1857. Epub 2011 May 18.
Abstract
Most scoring assays for yeast prions are dependent on specific genetic markers and constructs that differ for each prion. Here we describe a simple colour assay for the [URE3] prion that works in the 74D-964 strain frequently used to score the [PSI(+)] prion. Although this assay can only be used to score for [URE3] in the [psi(-)] version of the strain, it makes it easier to examine the effects of host mutations or environmental changes on [URE3] or [PSI(+)] using a colour assay in the identical genetic background.
摘要
大多数用于酵母朊病毒的评分检测依赖于特定的遗传标记和构建体，每个朊病毒都不同。在这里，我们描述了一种用于 [URE3] 朊病毒的简单显色检测方法，该方法适用于经常用于评分 [PSI(+)] 朊病毒的 74D-964 菌株。尽管该检测方法只能用于在菌株的 [psi(-)] 版本中评分 [URE3]，但它使得使用相同的遗传背景中的显色检测更容易研究宿主突变或环境变化对 [URE3] 或 [PSI(+)] 的影响。
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