Harbour J W, Lai S L, Gazdar A F, Minna J D, Kaye F J
NCI-Navy Medical Oncology Branch, NIH, Bethesda, Maryland 20814.
Anticancer Res. 1990 Jan-Feb;10(1):23-7.
The short arm of chromosome 3 undergoes genetic loss in virtually all small cell lung cancers (SCLC). The most frequently deleted region includes the DNF15S2 locus (mapped to 3p21), suggesting that a putative recessive SCLC gene might be located nearby. Using a DNA probe subcloned from the DNF15S2 locus, we identified a 3.0 kb mRNA species and isolated corresponding partial cDNA clones. Analysis of the nucleic acid sequence showed it to be the same as a recently published transcribed gene isolated from this chromosomal 3p21 region. To determine whether this gene might represent a "recessive oncogene" we examined a large number of SCLC and other human tumors and found no evidence for DNA rearrangements or inactivation of RNA expression. We discuss the significance of this transcribed gene and the DNF15S2 locus in tumorigenesis.
在几乎所有的小细胞肺癌（SCLC）中，3号染色体短臂都会发生基因缺失。最常缺失的区域包括DNF15S2位点（定位于3p21），这表明一个假定的隐性SCLC基因可能位于附近。使用从DNF15S2位点亚克隆的DNA探针，我们鉴定出一种3.0 kb的mRNA种类，并分离出相应的部分cDNA克隆。核酸序列分析表明，它与最近从该3号染色体p21区域分离出的一个已发表的转录基因相同。为了确定该基因是否可能代表一个“隐性癌基因”，我们检测了大量的SCLC和其他人类肿瘤，未发现DNA重排或RNA表达失活的证据。我们讨论了这个转录基因和DNF15S2位点在肿瘤发生中的意义。
