灵长类动物的p53在体外抑制SV40 DNA复制。

Primate's p53 inhibits SV40 DNA replication in vitro.

作者信息

Miyamoto N, Kihara E, Inada T, Katsura S, Murakami Y

机构信息

Tsukuba Life Sci. Center, Inst. of Phys. and Chem. Res., Ibaraki, Japan.

出版信息

Biochem Biophys Res Commun. 1990 Apr 30;168(2):604-8. doi: 10.1016/0006-291x(90)92363-5.

DOI:10.1016/0006-291x(90)92363-5

PMID:2159296

Abstract

Previous reports indicated that rodent p53 inhibits simian virus 40 (SV40) DNA replication in vitro as well as in vivo while that from primate cells does not (1-4). Here we report the evidence that p53 of primate origin also inhibits SV40 DNA replication in vitro. p53-SV40 large tumor antigen (T antigen) complex purified from SV40 infected COS-1 cells had little replication activity and inhibited SV40 DNA replication in vitro. These results suggest that inhibition of SV40 DNA replication by p53 should be regarded as general property of the protein and does not determine the mode of species specific replication of SV40 DNA.

摘要

先前的报告表明，啮齿动物的p53在体外和体内均能抑制猿猴病毒40（SV40）的DNA复制，而灵长类细胞的p53则不能（1-4）。在此，我们报告灵长类来源的p53在体外也能抑制SV40 DNA复制的证据。从感染SV40的COS-1细胞中纯化得到的p53-SV40大T抗原（T抗原）复合物几乎没有复制活性，并且在体外能抑制SV40 DNA复制。这些结果表明，p53对SV40 DNA复制的抑制作用应被视为该蛋白的普遍特性，而不能决定SV40 DNA的物种特异性复制模式。