Cohen R W, Campagnoni A T, Hull C D
Mental Retardation Research Center, University of California, Los Angeles.
Dev Neurosci. 1990;12(2):81-8. doi: 10.1159/000111837.
We assessed the ontogeny of murine voltage-dependent calcium channels by extracting mRNA from brains of mice at different postnatal ages and injecting the mRNA into oocytes of the frog, Xenopus laevis. Voltage-dependent Ca2(+)-activated Cl- channels were measured to assess the presence of Ca2(+) channels. When compared with water-injected oocytes (controls), an increase in Ca2(+) channels was not detected until postnatal day 7. The number of Ca2+ channels peaked between 9 and 18 days and began to decline by 35 days. Bath application of barium, serotonin and the Ca2+ channel antagonist, verapamil, to mRNA-injected oocytes confirmed the presence of Ca2+ channels.
我们通过从不同出生后年龄的小鼠大脑中提取mRNA，并将其注射到非洲爪蟾的卵母细胞中，来评估小鼠电压依赖性钙通道的个体发生。通过测量电压依赖性Ca2(+)激活的Cl-通道来评估Ca2(+)通道的存在。与注射水的卵母细胞（对照）相比，直到出生后第7天才检测到Ca2(+)通道增加。Ca2+通道数量在9至18天达到峰值，并在35天时开始下降。向注射mRNA的卵母细胞中浴用钡、血清素和Ca2+通道拮抗剂维拉帕米，证实了Ca2+通道的存在。
