Department of Food Science, University of Arkansas, Fayetteville, AR 72704, USA.
J Sci Food Agric. 2011 Aug 15;91(10):1920-4. doi: 10.1002/jsfa.4464. Epub 2011 May 18.
Shrimp wastes contain high-quality protein that is underutilized, and particularly peptides derived from shrimp wastes (normally used as animal feed) have not been utilized for bioactive properties. Hence the objective was to utilize shrimp waste proteins in generating peptides and to investigate these for cancer antiproliferative activities. The objectives involved hydrolyzing shrimp proteins (intact in shell) using a food-grade Cryotin enzyme, obtaining gastrointestinal resistant peptides, fractionation to generate < 10, 10-30 and > 30 kDa fractions, and evaluating for colon and liver cancer cell growth inhibitory effects. Three shrimp shells--whole langostino lobster shells from El Salvador (South America), shrimp shells from St Petersburg, FL (USA), and shrimp shell whites from the Gulf of Mexico, LA (USA)--were evaluated for the study.
Peptide fractions (<10 and 10-30 kDa) obtained from shrimp shell whites (Gulf of Mexico) as well as from langostino shells (El Salvador) significantly inhibited the growth of both colon and liver cancer cells by 60%, while < 10 kDa fraction from shrimp shells (FL) inhibited growth of liver cancer cells alone by 55%, compared to controls.
The promising anticancer peptide fractions from shrimp waste proteins has the potential for novel nutraceutical ingredient applications.
虾废物含有高质量的蛋白质，但未得到充分利用，特别是来自虾废物（通常用作动物饲料）的肽，尚未用于生物活性特性。因此，本研究的目的是利用虾废物蛋白生成肽，并研究这些肽的抗癌增殖活性。该研究涉及使用食品级 Cryotin 酶水解虾蛋白（带壳完整），获得具有胃肠道抗性的肽，进行分级分离以生成<10、10-30 和>30 kDa 的级分，并评估其对结肠和肝癌细胞生长的抑制作用。本研究评估了三种虾壳 - 来自萨尔瓦多（南美洲）的完整蓝龙虾壳、来自佛罗里达州圣彼得堡的虾壳以及来自美国路易斯安那州墨西哥湾的虾壳白。
来自虾壳白（墨西哥湾）和蓝龙虾壳（萨尔瓦多）的肽级分（<10 和 10-30 kDa）显著抑制结肠和肝癌细胞的生长，抑制率均为 60%，而来自佛罗里达州虾壳的<10 kDa 级分单独抑制肝癌细胞的生长，抑制率为 55%，与对照组相比。
虾废物蛋白的有前途的抗癌肽级分有可能成为新型营养成分应用。
