Department of Zoology, University of Madras, Life Science, Guindy Campus, Chennai, 600025, Tamilnadu, India.
Department of Environmental and Public Health, College of Health Sciences, Abu Dhabi University, Abu Dhabi, P.O. Box 59911, United Arab Emirates.
Cell Biochem Biophys. 2024 Mar;82(1):247-257. doi: 10.1007/s12013-023-01209-1. Epub 2024 Jan 6.
The present study aimed to investigate the purified protein from the epidermal mucus of marine catfish Tachysurus dussumieri on the human colon cancer cell line. The bioactive protein was purified with the Anion exchange chromatography and the collected fractions were then tested to assess cell viability in HT 29 cells through the MTT assay. The most responding active purified protein fraction (PPF III) was characterized with the MALDI-TOF/MS it shared a similar homology and sequence with 90% of antimicrobial peptides from external secretions of amphibians. Typical morphological changes of apoptotic cells, including cell shrinkage and detachment, DNA damage, and nuclear condensation were observed after the treatment of bioactive protein. PPF III triggered ROS, increasing the LDH activity, disruption of mitochondrial membrane potential, and upregulation of Cleaved caspase 3/9, Cytochrome-c, Bax, and downregulation of Bcl-2 protein and gene expression on HT 29 cells.
本研究旨在探讨海洋鲶鱼 Tachysurus dussumieri 表皮黏液中的纯化蛋白对人结肠癌细胞系的影响。采用阴离子交换层析法对生物活性蛋白进行纯化，收集的馏分通过 MTT assay 评估其对 HT 29 细胞活力的影响。响应性最强的活性纯化蛋白部分（PPF III）通过 MALDI-TOF/MS 进行表征，其与来自两栖动物外分泌液的 90%抗菌肽具有相似的同源性和序列。在生物活性蛋白处理后，观察到典型的凋亡细胞形态学变化，包括细胞收缩和脱落、DNA 损伤和核浓缩。PPF III 触发 ROS 增加，LDH 活性升高，线粒体膜电位破坏，Cleaved caspase 3/9、Cytochrome-c、Bax 上调，Bcl-2 蛋白和基因表达下调。
