Department of Chemistry, Polymer Research Institute, and BK School of Molecular Science, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea.
Biomacromolecules. 2011 Jul 11;12(7):2822-33. doi: 10.1021/bm200572t. Epub 2011 May 27.
Two new DNA-mimicking brush polymers were synthesized: poly[oxy(11-(3-(9-adeninyl)propionato)-undecanyl-1-thiomethyl)ethylene] (PECH-AP) and poly[oxy(11-(5-(9-adenylethyloxy)-4-oxopentanoato)undecanyl-1-thiomethyl)ethylene] (PECH-AS). These polymers were found to be thermally stable up to 220 °C and could be applied easily by conventional coating processes to produce good quality films. Interestingly, both brush polymers formed molecular multibilayer structures to provide an adenine-rich surface. Despite the structural similarities, PECH-AS surprisingly exhibited higher hydrophilicity and better water sorption properties than PECH-AP. These differences were attributed to the chemical structures in the bristles of the polymers. The adenine-rich surfaces of the polymer films demonstrated selective protein adsorption, suppressed bacterial adherence, facilitated HEp-2 cell adhesion, and exhibited good biocompatibility in mice. However, the high hydrophilicity and good water sorption characteristics of the PECH-AS film suggest that this brush polymer is better suited to applications requiring good biocompatibility and reduced chance of bacterial infection compared with the PECH-AP film.
合成了两种新的 DNA 模拟刷状聚合物：聚[氧（11-（3-（9-腺嘌呤基）丙酸盐）-十一烷-1-硫甲基）乙烯]（PECH-AP）和聚[氧（11-（5-（9-腺嘌呤乙氧基）-4-氧代戊酸）十一烷-1-硫甲基）乙烯]（PECH-AS）。这些聚合物在 220°C 以下表现出热稳定性，并且可以通过常规涂覆工艺轻松应用，以生产高质量的薄膜。有趣的是，两种刷状聚合物都形成了分子多层结构，提供了富含腺嘌呤的表面。尽管结构相似，但 PECH-AS 出人意料地表现出比 PECH-AP 更高的亲水性和更好的吸水性。这些差异归因于聚合物刷毛中的化学结构。聚合物薄膜的富含腺嘌呤的表面表现出选择性蛋白质吸附、抑制细菌附着、促进 HEp-2 细胞黏附以及在小鼠中表现出良好的生物相容性。然而，与 PECH-AP 薄膜相比，PECH-AS 薄膜的高亲水性和良好的吸水性特征表明，这种刷状聚合物更适合需要良好生物相容性和降低细菌感染机会的应用。
