College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, P.R. China.
Pharm Biol. 2011 Oct;49(10):1091-6. doi: 10.3109/13880209.2011.568507. Epub 2011 May 20.
Polygonum amplexicaule D. Don var. sinense Forb. (Polygonaceae) (PAF) is a well known traditional herb used to treat some diseases, such as fractures, rheumatoid arthritis, muscle injury, and pain. However, its pharmacological mechanism of promoting the healing of fractures is still unknown.
The present study was designed to investigate the effects of PAF ethanol extracts on the proliferation and differentiation of osteoblastic MC3T3-E1 cell in vitro, thereby to illuminate the pharmacological mechanism to promote the healing of fractures.
The effects of PAF ethanol extracts on MC3T3-E1 cell proliferation and differentiation were detected by using CCK-8, cell cycle, alkaline phosphatase (ALP), and prostaglandin E(2) (PGE(2)) assays in vitro.
The results showed that PAF ethanol extracts significantly stimulated cell proliferation at 0.1-100 μg/mL and the proportion of cells in S-phase increased from 16.33 to 27.29% in osteoblastic MC3T3-E1 cells. Moreover, PAF ethanol extracts increased ALP expression in MC3T3-E1 cells at the concentration from 0.1 to 100 μg/mL and inhibited PGE(2) production induced by TNF-α in osteoblasts at the concentrations ranging from 10 to 100 μg/mL in MC3T3-E1 osteoblasts.
These results indicated that PAF directly stimulates cell proliferation and differentiation of osteoblasts; therefore, this study preliminarily explored the pharmacological mechanism of PAF to promote the healing of bone rheumatism and various fractures.
Polygonum amplexicaule D. Don var. sinense Forb.（Polygonaceae）（PAF）是一种广为人知的传统草药，用于治疗骨折、类风湿关节炎、肌肉损伤和疼痛等疾病。然而，其促进骨折愈合的药理机制尚不清楚。
本研究旨在探讨 PAF 乙醇提取物对体外成骨细胞 MC3T3-E1 增殖和分化的影响，从而阐明促进骨折愈合的药理机制。
采用 CCK-8、细胞周期、碱性磷酸酶（ALP）和前列腺素 E（2）（PGE（2））检测法，体外检测 PAF 乙醇提取物对 MC3T3-E1 细胞增殖和分化的影响。
结果表明，PAF 乙醇提取物在 0.1-100μg/ml 浓度范围内显著刺激细胞增殖，成骨细胞 MC3T3-E1 中细胞 S 期比例从 16.33%增加到 27.29%。此外，PAF 乙醇提取物在 0.1-100μg/ml 浓度范围内增加了 MC3T3-E1 细胞中的 ALP 表达，并在 10-100μg/ml 浓度范围内抑制了 TNF-α诱导的 MC3T3-E1 成骨细胞中 PGE（2）的产生。
这些结果表明，PAF 直接刺激成骨细胞的增殖和分化；因此，本研究初步探讨了 PAF 促进骨风湿和各种骨折愈合的药理机制。
