Facultad de Química, Departamento de Farmacia, Universidad Nacional Autónoma de México, Ciudad Universitaria Coyoacan, México D.F., México.
Pharm Biol. 2011 Oct;49(10):1052-7. doi: 10.3109/13880209.2011.564634. Epub 2011 May 20.
The main use of stem bark infusions of Alnus acuminata ssp. arguta (Schlecht.) Furlow (Betulaceae) includes treatments for acute inflammation in Mexican traditional medicine.
n-Hexane (CHE), chloroform (CCE), and methanol (CME) extracts of the stem bark were investigated for anti-inflammatory activity and its safety.
The anti-inflammatory effects of the orally administered CME, CCE, and CHE extracts, using carrageenan-induced rat hind paw edema model, and acute oral toxicity in mice, using Lorke's method, were determined.
The column chromatographic fraction (CME-3) showed a higher anti-inflammatory activity (92.2%) (IC(50): 60.8 mg/mL) as compared with CME (76.9%); both were in the same order of magnitude as that of indomethacin, the positive control drug. Safety parameters for acute oral toxicity test showed that CME was not toxic (LD(50): >5000). Several triterpenoids (1-7) from hexane extracts and diarylheptanoids (10-14) from methanol extracts of A. acuminata ssp. arguta were isolated and characterized.
These results confirm the traditional uses of A. acuminata in acute inflammatory conditions and its safety for consumption.
桤木亚种（Schlecht.）Furlow（桦木科）的茎皮浸剂主要用于治疗墨西哥传统医学中的急性炎症。
研究了该种茎皮的正己烷（CHE）、氯仿（CCE）和甲醇（CME）提取物的抗炎活性及其安全性。
采用角叉菜胶诱导的大鼠后足肿胀模型，通过 Lorke 法测定口服给予 CME、CCE 和 CHE 提取物的抗炎作用，以及急性口服毒性。
柱色谱分离得到的 CME 馏分（CME-3）表现出较高的抗炎活性（92.2%）（IC50：60.8mg/mL），与 CME（76.9%）相当，与阳性对照药物吲哚美辛相当。急性口服毒性试验的安全性参数表明，CME 无毒性（LD50：>5000）。从桤木亚种的正己烷提取物中分离并鉴定了几种三萜类化合物（1-7），从甲醇提取物中分离并鉴定了二芳基庚烷类化合物（10-14）。
这些结果证实了桤木亚种在急性炎症情况下的传统用途及其可食用的安全性。
