通过流式细胞术对编码细胞内抗原的逆转录病毒载体进行快速滴定。
Rapid titration of retroviral vectors encoding intracellular antigens by flow cytometry.
作者信息
Sladek T L, Jacobberger J W
机构信息
Department of Genetics, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
出版信息
J Virol. 1990 Jun;64(6):3135-8. doi: 10.1128/JVI.64.6.3135-3138.1990.
Abstract
Flow cytometry was used to detect cells infected with retroviral vectors encoding both simian virus 40 large T antigen and G418 resistance after indirect immunofluorescence staining using a T-antigen-specific monoclonal antibody and a fluorescein-conjugated secondary antibody. Titers of viral stocks determined by flow cytometry were equivalent to those determined by quantitation of G418-resistant colonies.
摘要
在使用T抗原特异性单克隆抗体和荧光素偶联二抗进行间接免疫荧光染色后，采用流式细胞术检测感染了编码猿猴病毒40大T抗原和G418抗性的逆转录病毒载体的细胞。通过流式细胞术测定的病毒原液滴度与通过定量G418抗性菌落所测定的滴度相当。
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