• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Rapid titration of retroviral vectors encoding intracellular antigens by flow cytometry.通过流式细胞术对编码细胞内抗原的逆转录病毒载体进行快速滴定。
J Virol. 1990 Jun;64(6):3135-8. doi: 10.1128/JVI.64.6.3135-3138.1990.
2
Using retroviral vectors to express SV40 tumor antigens.
Methods Mol Biol. 2001;165:129-50. doi: 10.1385/1-59259-117-5:129.
3
Establishment of a highly differentiated immortalized adult human hepatocyte cell line by retroviral gene transfer.
Transplant Proc. 2000 Nov;32(7):2368-9. doi: 10.1016/s0041-1345(00)01702-4.
4
Reversible immortalization of adult human hepatocytes(1).
Transplant Proc. 2000 Nov;32(7):2331-2. doi: 10.1016/s0041-1345(00)01686-9.
5
Monoclonal antibody analysis of simian virus 40 small t-antigen expression in infected and transformed cells.感染细胞和转化细胞中猿猴病毒40小t抗原表达的单克隆抗体分析
J Virol. 1989 Jul;63(7):3128-34. doi: 10.1128/JVI.63.7.3128-3134.1989.
6
A simple and rapid method for the determination of recombinant retrovirus titer by G418 selection.一种通过G418筛选测定重组逆转录病毒滴度的简单快速方法。
Gene Ther. 1996 Nov;3(11):1018-20.
7
Efficient immortalization of luminal epithelial cells from human mammary gland by introduction of simian virus 40 large tumor antigen with a recombinant retrovirus.通过重组逆转录病毒导入猿猴病毒40大肿瘤抗原高效永生化人乳腺腔上皮细胞。
Proc Natl Acad Sci U S A. 1991 May 1;88(9):3520-4. doi: 10.1073/pnas.88.9.3520.
8
Improved mammalian vectors for high expression of G418 resistance.
Cell Struct Funct. 1987 Dec;12(6):575-80. doi: 10.1247/csf.12.575.
9
Long-term transplantation of canine keratinocytes made resistant to G418 through retrovirus-mediated gene transfer.通过逆转录病毒介导的基因转移使犬角质形成细胞对遗传霉素（G418）产生抗性后的长期移植。
Proc Natl Acad Sci U S A. 1990 Mar;87(6):2349-53. doi: 10.1073/pnas.87.6.2349.
10
Persistence of the SV40 early region without expression in permissive simian cells.猿猴病毒40（SV40）早期区域在允许性猿猴细胞中持续存在但无表达。
Virus Res. 1994 Dec;34(3):237-63. doi: 10.1016/0168-1702(94)90126-0.

引用本文的文献

1
E2F-1 binding affinity for pRb is not the only determinant of the E2F-1 activity.E2F-1 与 pRb 的结合亲和力并不是决定 E2F-1 活性的唯一因素。
Int J Biol Sci. 2010 Jul 4;6(4):382-95. doi: 10.7150/ijbs.6.382.
2
E2F-1 has dual roles depending on the cell cycle.E2F-1 在细胞周期中具有双重作用。
Int J Biol Sci. 2010 Mar 3;6(2):116-28. doi: 10.7150/ijbs.6.116.
3
Growth, immortalization, and differentiation potential of normal adult human proximal tubule cells.正常成人近端肾小管细胞的生长、永生化及分化潜能
In Vitro Cell Dev Biol Anim. 2004 Jan-Feb;40(1-2):22-34. doi: 10.1290/1543-706X(2004)40<22:GIADPO>2.0.CO;2.
4
A subset of cells expressing SV40 large T antigen contain elevated p53 levels and have an altered cell cycle phenotype.表达SV40大T抗原的一部分细胞含有升高的p53水平，并具有改变的细胞周期表型。
Cell Prolif. 2000 Apr;33(2):115-25. doi: 10.1046/j.1365-2184.2000.00168.x.
5
Growth of anchorage-dependent mammalian cells on plastic petri dishes.贴壁依赖型哺乳动物细胞在塑料培养皿上的生长。
In Vitro Cell Dev Biol Anim. 1995 Jun;31(6):430-1. doi: 10.1007/BF02634254.
6
Overexpression of the E2F-1 transcription factor gene mediates cell transformation.E2F-1转录因子基因的过表达介导细胞转化。
Gene Expr. 1995;4(4-5):195-204.
7
Retrovirus-mediated gene transfer to cystic fibrosis airway epithelial cells: effect of selectable marker sequences on long-term expression.逆转录病毒介导的基因转移至囊性纤维化气道上皮细胞：选择标记序列对长期表达的影响。
Nucleic Acids Res. 1993 Feb 11;21(3):663-9. doi: 10.1093/nar/21.3.663.
8
Mechanism of entry of a xenotropic MMuLV-derived recombinant retrovirus into porcine cells using the expression of the reporter nlslacZ gene.
Arch Virol. 1992;124(1-2):57-67. doi: 10.1007/BF01314625.
9
Simian virus 40 large T-antigen expression decreases the G1 and increases the G2 + M cell cycle phase durations in exponentially growing cells.猿猴病毒40大T抗原的表达缩短了指数生长细胞的G1期，并延长了G2+M期的细胞周期时长。
J Virol. 1992 Feb;66(2):1059-65. doi: 10.1128/JVI.66.2.1059-1065.1992.

本文引用的文献

1
Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus.逆转录病毒包装突变体的构建及其用于产生无辅助病毒的缺陷型逆转录病毒。
Cell. 1983 May;33(1):153-9. doi: 10.1016/0092-8674(83)90344-6.
2
Construction and applications of a highly transmissible murine retrovirus shuttle vector.一种高传染性小鼠逆转录病毒穿梭载体的构建与应用
Cell. 1984 Jul;37(3):1053-62. doi: 10.1016/0092-8674(84)90440-9.
3
Adsorption of Rous sarcoma virus to genetically susceptible and resistant chicken cells studied by laser flow cytometry.通过激光流式细胞术研究劳氏肉瘤病毒对遗传易感和抗性鸡细胞的吸附作用。
J Virol. 1982 Mar;41(3):958-64. doi: 10.1128/JVI.41.3.958-964.1982.
4
Uniform nomenclature for monoclonal antibodies directed against virus-coded proteins of simian virus 40 and polyoma virus.针对猴病毒40和多瘤病毒的病毒编码蛋白的单克隆抗体的统一命名法。
J Virol. 1982 Feb;41(2):709. doi: 10.1128/JVI.41.2.709-709.1982.
5
Monoclonal antibodies specific for simian virus 40 tumor antigens.针对猴病毒40肿瘤抗原的单克隆抗体。
J Virol. 1981 Sep;39(3):861-9. doi: 10.1128/JVI.39.3.861-869.1981.
6
Enhancement and inhibition of avian sarcoma viruses by polycations and polyanions.聚阳离子和聚阴离子对禽肉瘤病毒的增强和抑制作用。
Virology. 1969 Jul;38(3):414-26. doi: 10.1016/0042-6822(69)90154-8.
7
Redesign of retrovirus packaging cell lines to avoid recombination leading to helper virus production.逆转录病毒包装细胞系的重新设计，以避免重组导致辅助病毒产生。
Mol Cell Biol. 1986 Aug;6(8):2895-902. doi: 10.1128/mcb.6.8.2895-2902.1986.
8
Evidence that the packaging signal of Moloney murine leukemia virus extends into the gag region.莫洛尼鼠白血病病毒包装信号延伸至gag区域的证据。
J Virol. 1987 May;61(5):1639-46. doi: 10.1128/JVI.61.5.1639-1646.1987.
9
Construction of a defective retrovirus containing the human hypoxanthine phosphoribosyltransferase cDNA and its expression in cultured cells and mouse bone marrow.一种含有人类次黄嘌呤磷酸核糖转移酶互补脱氧核糖核酸的缺陷型逆转录病毒的构建及其在培养细胞和小鼠骨髓中的表达。
Mol Cell Biol. 1987 Feb;7(2):854-63. doi: 10.1128/mcb.7.2.854-863.1987.
10
Factors involved in production of helper virus-free retrovirus vectors.无辅助病毒逆转录病毒载体生产中涉及的因素。
Somat Cell Mol Genet. 1986 Mar;12(2):175-83. doi: 10.1007/BF01560664.

通过流式细胞术对编码细胞内抗原的逆转录病毒载体进行快速滴定。

Rapid titration of retroviral vectors encoding intracellular antigens by flow cytometry.

作者信息

Sladek T L, Jacobberger J W

机构信息

Department of Genetics, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.

出版信息

J Virol. 1990 Jun;64(6):3135-8. doi: 10.1128/JVI.64.6.3135-3138.1990.

DOI:10.1128/JVI.64.6.3135-3138.1990
PMID:2159569
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC249510/
Abstract

Flow cytometry was used to detect cells infected with retroviral vectors encoding both simian virus 40 large T antigen and G418 resistance after indirect immunofluorescence staining using a T-antigen-specific monoclonal antibody and a fluorescein-conjugated secondary antibody. Titers of viral stocks determined by flow cytometry were equivalent to those determined by quantitation of G418-resistant colonies.

摘要

在使用T抗原特异性单克隆抗体和荧光素偶联二抗进行间接免疫荧光染色后，采用流式细胞术检测感染了编码猿猴病毒40大T抗原和G418抗性的逆转录病毒载体的细胞。通过流式细胞术测定的病毒原液滴度与通过定量G418抗性菌落所测定的滴度相当。