Laboratório de Biomateriais, Universidade Federal de Itajubá (UNIFEI), Itajubá-MG, Brazil.
Artif Organs. 2011 May;35(5):471-7. doi: 10.1111/j.1525-1594.2011.01257.x.
Today, nanobiomaterials represent a very important class of biomaterials because they differ dramatically in their bulk precursors. The properties of these materials are determined by the size and morphology, thus creating a fascinating line in their physicochemical properties. Polyaniline nanotubes (PANINTs) are one of the most promising nanobiomaterials for cardiac tissue engineering applications due to their electroactive properties. The biocompatibility and low hydrophilic properties of PANINTs can be improved by their functionalization with the highly hydrophilic polyglycerol dendrimers (PGLDs). Hydrophilicity plays a fundamental role in tissue regeneration and fundamental forces that govern the process of cell adhesion and proliferation. In this work, the biocompatible properties and cardiomyocyte proliferation onto PANINTs modified by PGLD are described. PGLDs were immobilized onto PANINTs via surface-initiated anionic ring-opening polymerization of glycidol. The microstructure and morphology of PGLD-PANINTs was determined by X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM), respectively. The cardiac cell growth on the PGLD-PANINTs was investigated. The PGLD-coated PANINTs showed noncytotoxic effects to Chinese hamster ovary cells. It was observed that the application of microcurrent stimulates the differentiation of cardiac cells cultured on PGLD-PANINTs scaffolds. The electroactive and biocompatible results of PGLD-PANINTs observed in this work demonstrate the potential of this nanobiomaterial for the culture of cardiac cells and open the possibility of using this material as a biocompatible electroactive three-dimensional matrix in cardiac tissue engineering.
今天，纳米生物材料代表了一类非常重要的生物材料，因为它们在其大块前体中差异巨大。这些材料的性质由其尺寸和形态决定，从而在其物理化学性质上形成了一条引人入胜的线。聚苯胺纳米管（PANINTs）是最有前途的用于心脏组织工程应用的纳米生物材料之一，因为它们具有电活性。通过与高度亲水性的聚甘油树枝状大分子（PGLD）进行功能化，可以改善 PANINTs 的生物相容性和低亲水性。亲水性在组织再生和控制细胞黏附和增殖过程的基本力中起着根本作用。在这项工作中，描述了通过 PGLD 修饰的 PANINTs 的生物相容性和心肌细胞增殖。通过环氧丙烷的表面引发阴离子开环聚合将 PGLD 固定在 PANINTs 上。通过 X 射线光电子能谱（XPS）和扫描电子显微镜（SEM）分别确定了 PGLD-PANINTs 的微观结构和形态。研究了 PGLD-PANINTs 上的心脏细胞生长。PGLD 涂层的 PANINTs 对中国仓鼠卵巢细胞表现出非细胞毒性作用。观察到施加微电流刺激在 PGLD-PANINTs 支架上培养的心脏细胞的分化。在这项工作中观察到的 PGLD-PANINTs 的电活性和生物相容性结果表明了这种纳米生物材料用于培养心脏细胞的潜力，并为将该材料用作心脏组织工程中的生物相容的电活性三维基质开辟了可能性。
