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通过聚合酶链反应对社区获得性下呼吸道感染中的肺炎支原体进行快速诊断。

Rapid diagnosis of Mycoplasma pneumoniae by polymerase chain reaction in community-acquired lower respiratory tract infections.

作者信息

Kumar Surinder, Saigal Sanjeev R, Sethi G R

机构信息

Department of Microbiology, Maulana Azad Medical College, New Delhi - 110002, India.

出版信息

Trop Doct. 2011 Jul;41(3):160-2. doi: 10.1258/td.2011.100422. Epub 2011 May 19.

DOI:10.1258/td.2011.100422
PMID:21596845
Abstract

Two hundred children hospitalized for community-acquired lower respiratory tract infections (LRTIs) were investigated for Mycoplasma pneumoniae employing serological tests and a P1 adhesin gene-based polymerase chain reaction assay (PCR) on nasopharyngeal aspirates. Serological evidence of M. pneumoniae infection was observed in 68 (34%) patients and PCR was positive in 20 (10%) children. Together PCR and/or enzyme immuno assay detected M. pneumoniae in 71(35.5%) children. Our data underline the role of M. pneumoniae in Indian children with community-acquired LRTIs even in children aged < 24 months.

摘要

对200名因社区获得性下呼吸道感染（LRTIs）住院的儿童进行了调查，采用血清学检测和基于P1黏附素基因的聚合酶链反应分析（PCR）对鼻咽抽吸物进行肺炎支原体检测。68名（34%）患者有肺炎支原体感染的血清学证据，20名（10%）儿童PCR检测呈阳性。PCR和/或酶免疫测定共同检测出71名（35.5%）儿童感染肺炎支原体。我们的数据强调了肺炎支原体在印度社区获得性LRTIs儿童中所起的作用，即使是在年龄小于24个月的儿童中。

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