通过原位表面修饰对谷胱甘肽 S-转移酶标记的凝集素进行定向，以创建用于糖组学分析的扩展凝集素微阵列。

Orientation of GST-tagged lectins via in situ surface modification to create an expanded lectin microarray for glycomic analysis.

作者信息

Propheter Daniel C, Mahal Lara K

机构信息

Department of Chemistry and Biochemistry, University of Texas at Austin, 1 University Station A5300, Austin, TX 78712-0265, USA.

出版信息

Mol Biosyst. 2011 Jul;7(7):2114-7. doi: 10.1039/c1mb05047h. Epub 2011 May 20.

DOI:10.1039/c1mb05047h

PMID:21597618

Abstract

Herein we describe the orientation of GST-tagged lectins on NHS-activated slides via a one-step deposition of the protein and a glutathione (GSH) scaffold. This technology overcomes the need for a premade GSH-surface to orient GST-tagged proteins, enabling us to rapidly expand the analytical capacity of lectin microarrays through addition of oriented lectins, while maintaining lectin diversity.

摘要

在此，我们描述了通过蛋白质和谷胱甘肽（GSH）支架的一步沉积，将谷胱甘肽-S-转移酶（GST）标记的凝集素定向固定在N-羟基琥珀酰亚胺（NHS）活化的载玻片上。该技术克服了对预制GSH表面来定向GST标记蛋白质的需求，使我们能够通过添加定向凝集素快速扩展凝集素微阵列的分析能力，同时保持凝集素的多样性。