The Department of Chemistry, University of Miami, Coral Gables, FL 33146, USA.
Chem Commun (Camb). 2011 Jul 7;47(25):7242-4. doi: 10.1039/c1cc10714c. Epub 2011 May 20.
A direct conjugation of organophosphorus acid anhydrolase (OPAA) with CdS quantum dots was prepared via arrested precipitation within the enzyme matrix. The bio-conjugate was found not only to retain enzyme conformational structure but also to retain enzyme activity and be effective at detecting diisopropyl fluorophosphate (DFP) at the micro molar level.
通过在酶基质内的截流沉淀作用，将有机磷酸酐酶（OPAA）与 CdS 量子点直接连接起来。这种生物缀合物不仅保留了酶的构象结构，而且保留了酶的活性，并能有效地检测到微摩尔级别的二异丙基氟磷酸（DFP）。
