Yasui W, Kuniyasu H, Akama Y, Kitahara K, Nagafuchi A, Ishihara S, Tsukita S, Tahara E
HIROSHIMA UNIV,SCH MED,DEPT PATHOL 1,1-2-3 KASUMI,MINAMI KU,HIROSHIMA 734,JAPAN. NATL INST PHYSIOL SCI,DEPT INFORMAT PHYSIOL,OKAZAKI,AICHI 444,JAPAN.
Oncol Rep. 1995 Jan;2(1):111-7.
The expression of cell-cell adhesion molecule, E-cadherin and its associated proteins, alpha- and beta-catenins in human gastric carcinomas was examined by Western blotting. All the seven gastric carcinoma cell lines expressed E-cadherin except KATOIII, which was derived from pleural effusion of a scirrhous type stomach cancer or Borrmann's type-4 carcinoma. The expression of alpha-catenin was not detected in HSC43 derived from scirrhous carcinoma, while HSC39 expressed abnormal beta-catenin caused by genetic alteration. In gastric carcinoma cases, the levels of E-cadherin and alpha-catenin were significantly lower in poorly differentiated adenocarcinomas and scirrhous carcinomas when compared to other types of gastric carcinomas. Deeply invasive carcinomas expressed E-cadherin and alpha-catenin at lower levels. However, the expression level of alpha-catenin was not necessarily consistent with that of E-cadherin. One of 10 gastric carcinomas examined showed complete deletion of alpha-catenin gene in Southern blotting. beta-catenin was expressed at lower level in poorly differentiated adenocarcinomas than in well-differentiated adenocarcinomas. These findings suggest that reduction in the expression of E-cadherin and its associated molecules, catenins, is involved in the development and infiltrative growth of scirrhous type gastric carcinomas.
采用蛋白质免疫印迹法检测人胃癌细胞间黏附分子E-钙黏蛋白及其相关蛋白α-连环蛋白和β-连环蛋白的表达。除KATOIII外，所有七种胃癌细胞系均表达E-钙黏蛋白，KATOIII来源于硬癌型胃癌或Borrmann 4型癌的胸腔积液。在来源于硬癌的HSC43中未检测到α-连环蛋白的表达，而HSC39表达由基因改变引起的异常β-连环蛋白。在胃癌病例中，与其他类型的胃癌相比，低分化腺癌和硬癌中E-钙黏蛋白和α-连环蛋白的水平显著降低。浸润性深的癌组织中E-钙黏蛋白和α-连环蛋白的表达水平较低。然而，α-连环蛋白的表达水平不一定与E-钙黏蛋白的表达水平一致。在检测的10例胃癌中，有1例在Southern印迹法中显示α-连环蛋白基因完全缺失。低分化腺癌中β-连环蛋白的表达水平低于高分化腺癌。这些发现表明，E-钙黏蛋白及其相关分子连环蛋白表达的降低与硬癌型胃癌的发生和浸润性生长有关。
