Kiaris H, Ergazaki M, Sakkas S, Athanasiadou E, Spandidos D
NATL HELLEN RES FDN,INST BIOL RES & BIOTECHNOL,GR-11635 ATHENS,GREECE. UNIV CRETE,SCH MED,IRAKLION,GREECE. GEN HOSP NIKEAS,CYTOL LAB,PIRAEUS,GREECE.
Oncol Rep. 1995 Sep;2(5):769-71. doi: 10.3892/or.2.5.769.
Mutations in the ras family genes (K-ras mainly) represent a common event in lung tumorigenesis which is frequently associated with poor clinical outcome. In order to investigate whether K-ras mutations are detectable in cytological material obtained from patients with lung cancer, 37 cytological specimens (16 fine needle aspiration and 21 bronchoscopy) were assessed for codon 12 point mutations in the H-, K- and N-ras genes by combined polymerase chain reaction-restriction fragment length polymorphism. K-ras codon 12 point mutations were found in 8 out of 37 (22%) specimens while no mutations were found in the H-ras and N-ras genes. Mutations were found in 27% (3 out of 11) of adenocarcinomas while in squamous cell carcinomas the incidence of mutations was 18% (3 out of 17). In addition, a K-ras codon 12 point mutation was found in one (12%) among 8 small cell carcinomas and in the only Hodgkin's lymphoma with metastasis in the lung. Our results are in agreement with previous results that recognise high incidence of K-ras activation in lung carcinomas, and indicate that detection of mutant ras alleles is possible in cytological material.
ras家族基因（主要是K-ras）的突变是肺癌发生过程中的常见事件，常与不良临床预后相关。为了研究肺癌患者的细胞学材料中是否可检测到K-ras突变，采用聚合酶链反应-限制性片段长度多态性联合方法，对37份细胞学标本（16份细针穿刺标本和21份支气管镜检查标本）的H-ras、K-ras和N-ras基因第12密码子点突变进行了评估。37份标本中有8份（22%）检测到K-ras第12密码子点突变，而H-ras和N-ras基因未发现突变。腺癌中有27%（11份中的3份）检测到突变，鳞状细胞癌的突变发生率为18%（17份中的3份）。此外，8份小细胞癌中有1份（12%）以及唯一1例肺转移的霍奇金淋巴瘤中发现了K-ras第12密码子点突变。我们的结果与先前认识到肺癌中K-ras激活发生率高的结果一致，并表明在细胞学材料中检测突变的ras等位基因是可行的。
