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基于包囊形态和 DNA 结果,从银鸥(Larus argentatus)中鉴定出的 Sarcocystis sp. 为 Sarcocystis wobeseri。

Sarcocystis sp. from the herring gull (Larus argentatus) identity to Sarcocystis wobeseri based on cyst morphology and DNA results.

机构信息

Nature Research Centre, Vilnius, Lithuania.

出版信息

Parasitol Res. 2011 Dec;109(6):1603-8. doi: 10.1007/s00436-011-2421-5. Epub 2011 May 20.

Abstract

Having studied 11 herring gulls (Larus argentatus) Sarcocystis cysts were found in neck and leg muscles of 4 birds. One type of sarcocysts (cyst type I) that have a thin (∼1.0 μm), smooth, or slightly wavy cyst wall without clearly visible protrusions and small (6.0-8.0 μm) lancet- or banana-shaped cystozoites was identified by the light microscopy. Sarcocysts extracted from one herring gull were used for electron microscopy and DNA analysis. Ultrastructurally, Sarcocystis sp. from the herring gull had the same tissue cyst wall type-1 as S. calchasi, S. columbae, and S. wobeseri parasitizing in birds. According to first internal transcribed spacer (ITS-1) region, 18S rRNA and 28S rRNA gene sequences, Sarcocystis sp. from the herring gull belongs to S. wobeseri. Nevertheless, without evidences of cross-transmission experiment sarcocysts extracted from herring gull at present time are named as S. wobeseri-like.

摘要

在对 11 只银鸥(Larus argentatus)进行研究后,在 4 只鸟的颈部和腿部肌肉中发现了 Sarcocystis 囊肿。通过光学显微镜鉴定出一种囊型(囊型 I)的 Sarcocysts,其囊壁薄(约 1.0μm),光滑或略带波纹,无明显可见的突起和小(6.0-8.0μm)柳叶刀或香蕉形的囊内体。从一只银鸥中提取的 Sarcocysts 用于电子显微镜和 DNA 分析。超微结构上,来自银鸥的 Sarcocystis sp. 与寄生在鸟类中的 S. calchasi、S. columbae 和 S. wobeseri 具有相同的组织囊壁 1 型。根据内部转录间隔区 1(ITS-1)区域、18S rRNA 和 28S rRNA 基因序列,来自银鸥的 Sarcocystis sp. 属于 S. wobeseri。然而,在没有交叉传播实验证据的情况下,目前从银鸥中提取的囊型仍被命名为 S. wobeseri 样。

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