[Influence of cultivation temperature and Yersinia pestis fra-operon carriage on morphological features of Yersinia pseudotuberculosis].

AIM

Studies of influence of Yersinia pestis fra-operon carriage on morphological properties of Y. pseudotuberculosis recipient strain cells and colonies at different temperature and cultivation time.

MATERIALS AND METHODS

Cultures of Y. pseudotuberculosis isogenic variants were grown on solid nutrient medium at 4 - 6, 9 - 11, 26 - 28 and 36 - 38 degrees C. Indirect hemagglutination was used to determine F1 antigen production. Cytorefractometry was used to determine live cell percentage in colonies. Size and dividing cells percentage was evaluated by using phase-contrast microscope "Lyumam-I2".

RESULTS

Time period between appearance of micro-colonies and achievement of maximum colony size increased with cultivation temperature decrease. Size of fra+ and fra- variant colony was not significantly different for every temperature regiment used and was significantly lower at the temperature of 36 - 38 degrees C.

CONCLUSION

Temperature has a significant influence on population growth, colony size and form.