[Effects of cholecystokinin octapeptide on the contractile activity of guinea-pig colonic smooth muscles, L-type calcium currents and membrane potentials of myocytes].

OBJECTIVE

To investigate the effects and mechanism of cholecystokinin octapeptide (CCK-8S) on the contractile activity of smooth muscles, L-type calcium current and membrane potentials of proximal colon myocytes in guinea pig.

METHODS

(1) Strips of proximal colon were obtained from adult guinea pigs. The contraction of these stripes was measured by a RM6240 multi-channel physiological signal system. (2) Suspension of single smooth muscle cells (SMCs) were obtained from proximal colon and isolated by enzymatic digestion. The effect of CCK-8S on intracellular calcium concentration ([Ca(2+)]i) of SMCs was examined by fura-2-loaded microfluorimetric measurement. (3) Resting potential (RP), action potential (AP) and L-type calcium current (I(Ca-L)) were recorded by patch-clamp technique.

RESULTS

(1) The contractile amplitude and frequency of muscle stripes enhanced by CCK-8S (10(-7) mol/L) were (149 ± 12)% and (132 ± 13)% respectively of those of control group (all P < 0.05). They were significantly attenuated by pretreating strips with CCK1 receptor antagonist devazepide (10(-7) mol/L), L-type calcium channel blocker nifedipine (10(-5) mol/L), Ca(2+)-ATPase inhibitor TG (thapsigargin) (10(-5) mol/L) and BA (boric acid) (10(-5) mol/L) respectively. (2) [Ca(2+)]i of SMCs intensified by CCK-8S was (738 ± 24)% of that of control group. And it was inhibited by pretreating SMCs with devazepide (all P < 0.05). (3) After the superfusion of CCK-8S, RP depolarized to (52 ± 9)%, the exogenously stimulated peak values of AP rose to (140 ± 4)% and fast repolarization time of AP decreased to (61 ± 13)% (all P < 0.05). They were significantly inhibited when these cells were pretreated with devazepide and/or nifedipine (n = 8, P < 0.05 for each group) whereas CI 988 had little effect. (4) The CCK-8S-evoked I(Ca-L) of SMCs at the voltage of + 10 mV was boosted to (138 ± 7)%. Such an effect was suppressed by a pretreatment with nifedipine, devazepide, TG and BA respectively. In the presence of an inhibitor of inositol 1,4,5-trisphosphate (IP3) receptors, heparin (10(-6) mol/L) and an protein kinase C (PKC) inhibitor, saurosporine (10(-6) mol/L), CCK-8S did not significantly intensify I(Ca-L) (all P > 0.05).

CONCLUSION

CCK-8S promotes proximal colon contraction by CCK1 receptors through the activation of IP3-mediated PKC pathway.