Department of Obstetrics and Gynecology, College of Medicine, Konyang University, Daejeon 302-711, Korea.
Hum Immunol. 2011 Aug;72(8):621-6. doi: 10.1016/j.humimm.2011.03.013. Epub 2011 May 11.
Regulatory T (Treg) cells interact with B, natural killer (NK), and dendritic cells in addition to other T cells. In this study, we aimed at determining whether Foxp3(+) T cells and subpopulations have any correlation with other lymphocyte subsets and their functions in a systemic immune environment. Peripheral blood was drawn from 22 nonpregnant healthy women. T, B, and NK cell subpopulations were measured by immunophenotype analysis. Intracellular Foxp3, cytokine expression (tumor necrosis factor-α [TNF-α], interferon-γ [IFN-γ], and interleukin-10 [IL]-10), and NK-cell cytotoxicity were analyzed by flow cytometric analysis. Correlations between Foxp3(+) T cells and other immune variables were analyzed under control of age and menstrual phases. Foxp3(+), Foxp3(low), and CD4(+)Foxp3(+) cells significantly correlated with CD4(+)CD25(+), CD4(+)CD25(dim), and CD4(+)CD25(bright) cells. Foxp3(+), Foxp3(low), and CD4(+)Foxp3(+) cells positively correlated with CD3(+) and CD3(+)CD4(+) T cells, but negatively correlated with CD3(-)CD56(+) and CD3(-)CD56(dim) NK cells. CD4(+)Foxp3(high) Treg cells were positively correlated with CD3(+)CD4(+)TNF-α(+) (p = 0.014) and negatively correlated with CD3(+)CD8(+)IL-10(+) T cells (p = 0.001). The ratio of type 1/2 cytokine-producing CD3(+)CD8(+) cells demonstrated a positive correlation with CD4(+)Foxp3(high) cells (p ≤ 0.01). CD8(+)Foxp3(+) cells were positively correlated with CD3(+)CD4(+)IL-10(+) cells (p = 0.007) and negatively correlated with CD3(+)CD8(+)TNF-α(+) cells (p = 0.008). In conclusion, each Foxp3(+) Treg cell subpopulation has unique immune interaction, which controls particular subsets of lymphocytes.
调节性 T（Treg）细胞除了与其他 T 细胞相互作用外，还与 B 细胞、自然杀伤（NK）细胞和树突状细胞相互作用。在这项研究中，我们旨在确定 Foxp3(+)T 细胞及其亚群与其他淋巴细胞亚群及其在全身免疫环境中的功能是否存在任何相关性。从 22 名非妊娠健康女性中抽取外周血。通过免疫表型分析测量 T、B 和 NK 细胞亚群。通过流式细胞术分析测定细胞内 Foxp3、细胞因子表达（肿瘤坏死因子-α[TNF-α]、干扰素-γ[IFN-γ]和白细胞介素-10[IL]-10）和 NK 细胞细胞毒性。在控制年龄和月经周期的情况下，分析 Foxp3(+)T 细胞与其他免疫变量之间的相关性。Foxp3(+)、Foxp3(low)和 CD4(+)Foxp3(+)细胞与 CD4(+)CD25(+)、CD4(+)CD25(dim)和 CD4(+)CD25(bright)细胞显著相关。Foxp3(+)、Foxp3(low)和 CD4(+)Foxp3(+)细胞与 CD3(+)和 CD3(+)CD4(+)T 细胞呈正相关，与 CD3(-)CD56(+)和 CD3(-)CD56(dim)NK 细胞呈负相关。CD4(+)Foxp3(high)Treg 细胞与 CD3(+)CD4(+)TNF-α(+)呈正相关（p=0.014），与 CD3(+)CD8(+)IL-10(+)T 细胞呈负相关（p=0.001）。产生 1/2 型细胞因子的 CD3(+)CD8(+)细胞的比例与 CD4(+)Foxp3(high)细胞呈正相关（p≤0.01）。CD8(+)Foxp3(+)细胞与 CD3(+)CD4(+)IL-10(+)细胞呈正相关（p=0.007），与 CD3(+)CD8(+)TNF-α(+)细胞呈负相关（p=0.008）。总之，每个 Foxp3(+)Treg 细胞亚群都具有独特的免疫相互作用，控制着特定的淋巴细胞亚群。
