Department of Microbiology and Immunology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, USA.
Adv Virus Res. 2011;79:139-64. doi: 10.1016/B978-0-12-387040-7.00009-3.
Until recently, single-stranded negative sense RNA viruses (ssNSVs) were one of only a few important human viral pathogens, which could not be created from cDNA. The inability to manipulate their genomes hindered their detailed genetic analysis. A key paper from Conzelmann's laboratory in 1994 changed this with the publication of a method to recover rabies virus (RABV) from cDNA. This discovery not only dramatically changed the broader field of ssNSV biology but also opened a whole new avenue for studying RABV pathogenicity, developing novel RABV vaccines as well a new generation of RABV-based vaccine vectors, and creating research tools important in neuroscience such as neuronal tracing.
直到最近，单链负义 RNA 病毒 (ssNSVs) 还是少数几种重要的人类病毒病原体之一，它们不能从 cDNA 中产生。无法操纵它们的基因组阻碍了对其遗传的详细分析。1994 年，Conzelmann 实验室的一篇重要论文发表，该文公布了一种从 cDNA 中回收狂犬病病毒 (RABV) 的方法，改变了这一状况。这一发现不仅极大地改变了 ssNSV 生物学的更广泛领域，也为研究 RABV 的致病性、开发新型 RABV 疫苗和新一代基于 RABV 的疫苗载体开辟了全新的途径，并为神经科学中的研究工具（如神经元示踪）创造了重要条件。
