Liu Zhi-feng, Xu Juan, E Ling-ling, Wang Dong-sheng, Bei Dan-dan
Department of Stomatology, General Hospital of PLA, Beijing 100853, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2011 May;31(5):813-6.
To evaluate the effect of pulsed ultrasound on the expressions of osteoprotegerin (OPG) and receptor activator nuclear factor kappaB ligand (RANKL) during root resorption in a mouse model of orthodontic tooth movement.
Thirty-two male Wistar rats (6-8 weeks old) were randomly assigned into 4 equal groups, including the blank control group, two ultrasound exposure groups with daily local LIPUS stimulation (100 and 150 MW/cm(2)) for 10 days during mechanical loading, and the control group with mechanical loading but not LIPUS exposure. Nickel-titanium closed-coil springs were used to generate 100 g mesial force for 10 days to move the maxillary right first molars. The expression of OPG and RANKL proteins at the compression sites was detected by immunohistochemistry.
Ultrasound stimulation significantly up-regulated the expression of OPG and down-regulated RANKL expression (P<0.05). The expressions of OPG and RANKL showed significant differences between the two ultrasound exposure groups (P<0.05).
Ultrasound stimulation might be useful to protect against root resorption and accelerate its repair by regulating the expressions of OPG and RANKL.
在正畸牙移动的小鼠模型中，评估脉冲超声对牙根吸收过程中骨保护素（OPG）和核因子κB受体活化因子配体（RANKL）表达的影响。
将32只6-8周龄的雄性Wistar大鼠随机分为4组，包括空白对照组、两个超声暴露组（在机械加载期间每日进行局部低强度脉冲超声刺激，强度分别为100和150 mW/cm²，持续10天）以及机械加载但未接受低强度脉冲超声暴露的对照组。使用镍钛闭合曲弹簧产生100 g的近中力，持续10天以移动上颌右侧第一磨牙。通过免疫组织化学检测压缩部位OPG和RANKL蛋白的表达。
超声刺激显著上调了OPG的表达并下调了RANKL的表达（P<0.05）。两个超声暴露组之间OPG和RANKL的表达存在显著差异（P<0.05）。
超声刺激可能通过调节OPG和RANKL的表达来预防牙根吸收并加速其修复。
