Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019, USA.
Int J Syst Evol Microbiol. 2012 Apr;62(Pt 4):832-838. doi: 10.1099/ijs.0.024349-0. Epub 2011 May 20.
A novel anaerobic, moderately thermophilic, NaCl-requiring fermentative bacterium, strain OS1T, was isolated from oil production water collected from Alaska, USA. Cells were Gram-negative, non-motile, non-spore-forming rods (1.7-2.7×0.4-0.5 µm). The G+C content of the genomic DNA of strain OS1T was 46.6 mol%. The optimum temperature, pH and NaCl concentration for growth of strain OS1T were 55 °C, pH 7 and 10 g l(-1), respectively. The bacterium fermented D-fructose, D-glucose, maltose, D-mannose, α-ketoglutarate, L-glutamate, malonate, pyruvate, L-tartrate, L-asparagine, Casamino acids, L-cysteine, L-histidine, L-leucine, L-phenylalanine, L-serine, L-threonine, L-valine, inositol, inulin, tryptone and yeast extract. When grown on D-glucose, 3.86 mol hydrogen and 1.4 mol acetate were produced per mol substrate. Thiosulfate, sulfur and L-cystine were reduced to sulfide, and crotonate was reduced to butyrate with glucose as the electron donor. 16S rRNA gene sequence analysis indicated that strain OS1T was related to Anaerobaculum thermoterrenum (99.7 % similarity to the type strain), a member of the phylum Synergistetes. DNA-DNA hybridization between strain OS1T and A. thermoterrenum DSM 13490T yielded 68 % relatedness. Unlike A. thermoterrenum, strain OS1T fermented malonate, maltose, tryptone, L-leucine and L-phenylalanine, but not citrate, fumarate, lactate, L-malate, glycerol, pectin or starch. The major cellular fatty acid of strain OS1T was iso-C15:0 (91 % of the total). Strain OS1T also contained iso-C13:0 3-OH (3 %), which was absent from A. thermoterrenum, and iso-C13:0 (2 %), which was absent from Anaerobaculum mobile. On the basis of these results, strain OS1T represents a novel species of the genus Anaerobaculum, for which the name Anaerobaculum hydrogeniformans sp. nov. is proposed. The type strain is OS1T (=DSM 22491T=ATCC BAA-1850T). An emended description of the genus Anaerobaculum is also given.
一株新型的兼性厌氧、中温、嗜盐发酵细菌 OS1T 菌株，从美国阿拉斯加的采油水中分离得到。细胞呈革兰氏阴性，无动力，不形成孢子的杆状（1.7-2.7×0.4-0.5 µm）。OS1T 菌株的基因组 DNA 的 G+C 含量为 46.6 mol%。OS1T 菌株的最适生长温度、pH 值和 NaCl 浓度分别为 55°C、pH 值 7 和 10 g l(-1)。该菌发酵 D-果糖、D-葡萄糖、麦芽糖、D-甘露糖、α-酮戊二酸、L-谷氨酸、丙二酸、丙酮酸、L-酒石酸、L-天冬酰胺、复合氨基酸、L-半胱氨酸、L-组氨酸、L-亮氨酸、L-苯丙氨酸、L-丝氨酸、L-苏氨酸、L-缬氨酸、肌醇、菊粉、胰蛋白胨和酵母提取物。当以 D-葡萄糖为底物时，每摩尔底物产生 3.86 摩尔氢气和 1.4 摩尔乙酸盐。硫代硫酸盐、硫和 L-胱氨酸被还原为硫化物，而丁烯酸被还原为丁酸，葡萄糖作为电子供体。16S rRNA 基因序列分析表明，OS1T 菌株与 Anaerobaculum thermoterrenum（与模式菌株相似度为 99.7%）密切相关，后者属于互营杆菌门。OS1T 菌株与 Anaerobaculum thermoterrenum DSM 13490T 的 DNA-DNA 杂交产生 68%的同源性。与 Anaerobaculum thermoterrenum 不同的是，OS1T 菌株发酵丙二酸盐、麦芽糖、胰蛋白胨、L-亮氨酸和 L-苯丙氨酸，但不发酵柠檬酸盐、富马酸盐、乳酸盐、L-苹果酸盐、甘油、果胶或淀粉。OS1T 菌株的主要细胞脂肪酸是 iso-C15:0（占总脂肪酸的 91%）。该菌株还含有 iso-C13:0 3-OH（3%），而这在 Anaerobaculum thermoterrenum 中不存在，以及 iso-C13:0（2%），而这在 Anaerobaculum mobile 中不存在。基于这些结果，OS1T 菌株代表了 Anaerobaculum 属的一个新种，现提议将其命名为 Anaerobaculum hydrogeniformans sp. nov.。模式菌株为 OS1T（=DSM 22491T=ATCC BAA-1850T）。还给出了对 Anaerobaculum 属的修正描述。
