Department of Pharmacology, School of Pharmacy, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Pharmacol Rep. 2011;63(2):544-7. doi: 10.1016/s1734-1140(11)70521-7.
The effects of sphingosine-1-phosphate (S1P) on bronchial smooth muscle (BSM) contractility were investigated in naive mice. S1P had no effect on the basal tone of the isolated BSM tissues. However, in the presence of S1P (10(-6) M), the BSM contractions induced by acetylcholine (ACh) and endothelin-1 (ET-1) were significantly augmented: both the ACh and ET-1 concentration-response curves were significantly shifted to the left. In contrast, the pretreatment with S1P had no effect on the contractions induced by high K(+) depolarization. It is thus possible that S1P augments BSM contraction induced by the activation of G protein-coupled receptors.
研究了鞘氨醇-1-磷酸（S1P）对支气管平滑肌（BSM）收缩性的影响。S1P 对分离的 BSM 组织的基础张力没有影响。然而，在 S1P（10（-6）M）存在下，乙酰胆碱（ACh）和内皮素-1（ET-1）诱导的 BSM 收缩显著增强：ACh 和 ET-1 的浓度-反应曲线均显著向左移位。相反，S1P 的预处理对由高 K（+）去极化诱导的收缩没有影响。因此，S1P 可能增强了 G 蛋白偶联受体激活引起的 BSM 收缩。
