Nair A S, Ravindran R, Lakshmanan B, Kumar S S, Tresamol P V, Saseendranath M R, Senthilvel K, Rao J R, Tewari A K, Ghosh S
Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala.
Trop Biomed. 2011 Apr;28(1):68-75.
A cross-sectional study was conducted using 150 blood samples collected from apparently normal / healthy crossbred cattle of Northern Kerala, South India, for detection of haemoprotozoan infections using staining techniques (Giemsa and Acridine Orange) and specific PCR. Theileria like piroplasms and Babesia bigemina were the only protozoan organisms detected in blood smears. Polymerase chain reaction using specific primers revealed amplification of products specific for Trypanosoma evansi (34.6%), Theileria sp. other than T. annulata (16%) and B. bigemina (0.6%). The higher prevalence rate of Trypanosoma evansi indicated that the subclinical parasitism can be due to higher prevalence of tabanid flies. The study also revealed the presence of a theilerial piroplasm other than T. annulata in North Kerala, which needs further investigation.
采用横断面研究方法，从印度南部喀拉拉邦北部表面正常/健康的杂交牛采集150份血样，运用染色技术（吉姆萨染色和吖啶橙染色）及特异性聚合酶链反应（PCR）检测血液原虫感染情况。血涂片检测到的仅有的原虫生物为泰勒虫样梨形虫和双芽巴贝斯虫。使用特异性引物进行的聚合酶链反应显示，扩增出了特异于伊氏锥虫（34.6%）、除环形泰勒虫外的其他泰勒虫属（16%）和双芽巴贝斯虫（0.6%）的产物。伊氏锥虫的高流行率表明，亚临床寄生虫感染可能归因于虻蝇的高流行率。该研究还揭示了喀拉拉邦北部存在除环形泰勒虫外的一种泰勒虫梨形虫，这需要进一步调查。
