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对来自砂拉越古晋雷江的野生罗氏沼虾体内寄生虫和传染性皮下及造血组织坏死病毒感染情况的筛查。

Screening of parasitic and IHHNV infections in wild giant freshwater prawn Macrobrachium rosenbergii from Rejang River at Kuching, Sarawak.

作者信息

Kua Beng Chu, Choong F C, Hazreen Nita M K, Muhd Faizul H A H, Bhassu S, Imelda R R, Mohammed M

机构信息

National Fish Health Research Centre, NaFisH FRI Batu Maung, 11960 Batu Maung, Penang, Malaysia.

出版信息

Trop Biomed. 2011 Apr;28(1):85-9.

Abstract

A preliminary survey of parasitic and infectious hypodermal and haematopoietic necrosis virus (IHHNV) infections in giant freshwater prawn from the Damak Sea of Rejang River, Kuching, Sarawak was conducted. Symptoms of black spots/patches on the rostrum, carapace, pleopods or telson were observed in most of the 107 samples collected. Parasitic examination revealed sessiline peritrichs such as (Zoothamnium sp.), nematode larvae, gregarine stage and cocoon of leech with prevalences of 1.2%, 1.2%, 5% and 17% respectively. Under histopathological examination, changes like accumulation of hemocytes around hepatopancreatic tubules due to vibriosis, basophilic intranuclear inclusions in the epithelium and E-cell of hepatopancreatic tubules as a result of HPV were seen through the section. No positive infection of IHHNV was detected in 78 samples. As such, the wild giant freshwater prawns in Damak Sea of Rejang River in Kuching are IHHNV-free though infections of parvo-like virus and bacteria were seen in histopathology.

摘要

对来自砂拉越古晋雷江河达马克海的淡水大虾的寄生虫和传染性皮下及造血组织坏死病毒(IHHNV)感染情况进行了初步调查。在所采集的107个样本中,大多数都观察到了在额剑、头胸甲、腹肢或尾扇上出现黑斑/斑块的症状。寄生虫检查发现固着型周毛虫(如聚缩虫属)、线虫幼虫、簇虫期以及水蛭茧,其感染率分别为1.2%、1.2%、5%和17%。在组织病理学检查中,通过切片可以看到由于弧菌病导致血细胞在肝胰腺小管周围积聚、肝胰腺小管上皮和E细胞中出现嗜碱性核内包涵体等变化,这是由HPV引起的。在78个样本中未检测到IHHNV的阳性感染。因此,古晋雷江河达马克海的野生淡水大虾未感染IHHNV,不过在组织病理学检查中发现了细小病毒样病毒和细菌感染。

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