早期兔胚胎中钠/钾三磷酸腺苷酶α亚基及α亚基mRNA水平

Sodium/potassium adenosine triphosphatase alpha-subunit and alpha-subunit mRNA levels in early rabbit embryos.

作者信息

Gardiner C S, Grobner M A, Menino A R

机构信息

Department of Animal Science, Oregon State University, Corvallis 97331-6702.

出版信息

Biol Reprod. 1990 Mar;42(3):539-44. doi: 10.1095/biolreprod42.3.539.

DOI:10.1095/biolreprod42.3.539

PMID:2160296

Abstract

Sodium/potassium adenosine triphosphatase (Na+/K+ ATPase) and Na+/K+ ATPase mRNA content of rabbit embryos during preimplantation development were evaluated. Changes in Na+/K+ ATPase alpha-subunit content were detected with Western blotting using polyclonal antiserum against guinea pig Na+/K+ ATPase. Total RNA samples from rabbit embryos were analyzed by using Northern blots hybridized with random primer-labeled cDNA for Na+/K+ ATPase alpha-subunit from sheep kidney. Northern blots exhibited a single mRNA band (3.65 kilobases) in sheep kidneys and rabbit embryos. Between Day 4 and Day 6 of development, Na+/K+ ATPase alpha-subunit mRNA content increased 35-fold whereas Na+/K+ ATPase alpha-subunit content increased 22-fold. The similar increase in Na+/K+ ATPase alpha-subunit mRNA and alpha-subunit content in rabbit embryos suggests that Na+/K+ ATPase is partly regulated at the mRNA level during blastocyst expansion.

摘要

对家兔胚胎植入前发育过程中钠钾腺苷三磷酸酶（Na⁺/K⁺ ATP酶）及其mRNA含量进行了评估。使用针对豚鼠Na⁺/K⁺ ATP酶的多克隆抗血清，通过蛋白质免疫印迹法检测Na⁺/K⁺ ATP酶α亚基含量的变化。用与随机引物标记的绵羊肾脏Na⁺/K⁺ ATP酶α亚基cDNA杂交的Northern印迹法分析家兔胚胎的总RNA样本。Northern印迹法在绵羊肾脏和家兔胚胎中均显示出一条单一的mRNA条带（3.65千碱基）。在发育的第4天至第6天之间，Na⁺/K⁺ ATP酶α亚基mRNA含量增加了35倍，而Na⁺/K⁺ ATP酶α亚基含量增加了22倍。家兔胚胎中Na⁺/K⁺ ATP酶α亚基mRNA和α亚基含量的相似增加表明，在囊胚扩张过程中，Na⁺/K⁺ ATP酶部分受mRNA水平调控。