Moss Stephanie C, Bates Michael, Parrino Patrick E, Woods T Cooper
Ochsner J. 2007 Fall;7(3):133-6.
Analyses of vascular smooth muscle cell and endothelial cell function through tissue culture techniques are often employed to investigate the underlying mechanisms regulating cardiovascular disease. As diseases such as diabetes mellitus and chronic kidney disease increase a patient's risk of cardiovascular disease, the development of methods for examining the effects of these diseases on vascular smooth muscle cells and endothelial cells is needed. Commercial sources of endothelial cells and vascular smooth muscle cells generally provide minimal donor information and are in limited supply. This study was designed to determine if vascular smooth muscle cells and endothelial cells could be isolated from human internal mammary arteries obtained from donors undergoing coronary artery bypass graft surgery. As coronary artery bypass graft surgery is a commonly performed procedure, this method would provide a new source for these cells that when combined with the donor's medical history will greatly enhance our studies of the effects of complicating diseases on vascular biology. Internal mammary artery tissue was obtained from patients undergoing coronary artery bypass graft surgery. Through a simple method employing two separate tissue digestions, vascular smooth muscle cells and endothelial cells were isolated and characterized. The isolated vascular smooth muscle cells and endothelial cells exhibited the expected morphology and were able to be passaged for further analysis. The vascular smooth muscle cells exhibited positive staining for α-smooth muscle actin and the endothelial cells exhibited positive staining for CD31. The overall purity of the isolations was > 95%. This method allows for the isolation of endothelial cells and vascular smooth muscle cells from internal mammary arteries, providing a new tool for investigations into the interplay of vascular diseases and complicating diseases such as diabetes and kidney disease.
通过组织培养技术分析血管平滑肌细胞和内皮细胞功能，常用于研究调节心血管疾病的潜在机制。由于糖尿病和慢性肾病等疾病会增加患者患心血管疾病的风险，因此需要开发检测这些疾病对血管平滑肌细胞和内皮细胞影响的方法。内皮细胞和血管平滑肌细胞的商业来源通常提供的供体信息极少，且供应有限。本研究旨在确定是否可以从接受冠状动脉搭桥手术的供体的人乳内动脉中分离出血管平滑肌细胞和内皮细胞。由于冠状动脉搭桥手术是一种常见的手术，这种方法将为这些细胞提供一种新来源，当与供体的病史相结合时，将极大地加强我们对并发疾病对血管生物学影响的研究。乳内动脉组织取自接受冠状动脉搭桥手术的患者。通过一种采用两次单独组织消化的简单方法，分离并鉴定了血管平滑肌细胞和内皮细胞。分离出的血管平滑肌细胞和内皮细胞呈现出预期的形态，并且能够传代用于进一步分析。血管平滑肌细胞对α-平滑肌肌动蛋白呈阳性染色，内皮细胞对CD31呈阳性染色。分离物的总体纯度>95%。这种方法能够从乳内动脉中分离出内皮细胞和血管平滑肌细胞，为研究血管疾病与糖尿病和肾病等并发疾病之间的相互作用提供了一种新工具。
