大鼠小脑切片浦肯野细胞层γ-氨基丁酸（GABA）受体的定量分析。

Quantitative analysis of gamma-aminobutyric acid (GABA) receptors of Purkinje cell layer from rat cerebellar slices.

Hussain S, Bagust J, Gardner C R, Ward R A, Walker R J

Department of Neurophysiology, School of Biochemical and Physiological Sciences, University of Southampton, Swindon, Wiltshire, U.K.

Gen Pharmacol. 1990;21(3):355-64. doi: 10.1016/0306-3623(90)90837-c.

Abstract

Extracellular recordings were made from Purkinje cell layer of rat cerebellar slices. Cell activity was sensitive to both magnesium and manganese ions. 2. Glutamic and aspartic acids both excited cell activity while gamma-aminobutyric acid (GABA), muscimol, taurine, beta-alanine and delta-aminolaevulinic acid all inhibited activity. 3. The sensitivity to GABA varied with depth while no such effect was observed with muscimol. 4. Bicuculline methiodide, picrotoxin and pitrazepin blocked the action of muscimol with pA2 values of 5.92, 5.97 and 5.71 respectively. 5. The benzodiazepines flurazepam and RU 32007 both potentiated the GABA inhibition and this potentiation was blocked by Ro 15-1788, a benzodiazepine antagonist.

摘要

细胞外记录取自大鼠小脑切片的浦肯野细胞层。细胞活性对镁离子和锰离子均敏感。2. 谷氨酸和天冬氨酸均能激发细胞活性，而γ-氨基丁酸（GABA）、蝇蕈醇、牛磺酸、β-丙氨酸和δ-氨基乙酰丙酸均抑制细胞活性。3. 对GABA的敏感性随深度而异，而对蝇蕈醇未观察到此类效应。4. 甲碘化荷包牡丹碱、苦味毒和匹拉唑平分别以5.92、5.97和5.71的pA2值阻断蝇蕈醇的作用。5. 苯二氮䓬类药物氟西泮和RU 32007均增强GABA的抑制作用，且这种增强作用被苯二氮䓬类拮抗剂Ro 15-1788阻断。