Colaert Niklaas, Vandekerckhove Joël, Martens Lennart, Gevaert Kris
Department of Medical Protein Research, VIB, Ghent University, B-9000, Ghent, Belgium.
Methods Mol Biol. 2011;753:373-98. doi: 10.1007/978-1-61779-148-2_25.
MS-driven proteomics has evolved over the past two decades to a high tech and high impact research field. Two distinct factors clearly influenced its expansion: the rapid growth of an arsenal of instrument and proteomic techniques that led to an explosion of high quality data and the development of software tools to analyze and interpret these data which boosted the number of scientific discoveries. In analogy with the benchmarking of new instruments and proteomic techniques, such software tools must be thoroughly tested and analyzed. Recently, new tools were developed for automatic peptide quantification in quantitative proteomic experiments. Here we present a case study where the most recent and frequently used tools are analyzed and compared.
在过去二十年中，质谱驱动的蛋白质组学已发展成为一个高科技且具有高影响力的研究领域。有两个明显不同的因素对其扩展产生了影响：仪器和蛋白质组学技术库的迅速增长，这导致了高质量数据的激增；以及用于分析和解释这些数据的软件工具的开发，这推动了科学发现数量的增加。与新仪器和蛋白质组学技术的基准测试类似，此类软件工具必须经过全面测试和分析。最近，在定量蛋白质组学实验中开发了用于自动肽定量的新工具。在此，我们展示一个案例研究，对最新且常用的工具进行分析和比较。
