聚（ε-己内酯）/丝素蛋白纳米纤维支架的制备及其细胞相容性研究

[Preparation and cytocompatibility study of poly (epsilon-caprolactone)/silk sericin nanofibrous scaffolds].

作者信息

Li Haibin, Li Linhao, Qian Yuna, Cai Kaiyong, Lu Yonggang, Zhong Li, Liu Wanqian, Yang Li

机构信息

Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044, China.

出版信息

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2011 Apr;28(2):305-9.

PMID:21604491

Abstract

Three-dimensional poly (epsilon-caprolactone)/silk sericin (PCL/SS) porous nanofibrous scaffolds were prepared by electrospinning. The structure and properties of the scaffolds were characterized by Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM), Fourier Transform Infrared Spectroscopy (FTIR) and water contact angle instrument. Studies on cell adhension and proliferation were carried out by culturing human primary skin fibroblast cells (FEK4) on these scaffolds using SEM and MTS. The experimental results showed that the PCL/SS nanofibrous scaffolds with SS nanoparticles had porous non-woven mesh structure with nanofibrous cross-linked with each other. Fiber diameter was very uniform and precise, and the secondary structure of SS protein had not been changed. Furthermore, the capability of hydrophile increased with the SS addition, which improved FEK4 cells adhesion and proliferation on the scaffolds.

摘要

通过静电纺丝制备了三维聚（ε-己内酯）/丝素蛋白（PCL/SS）多孔纳米纤维支架。采用扫描电子显微镜（SEM）、透射电子显微镜（TEM）、傅里叶变换红外光谱（FTIR）和水接触角仪对支架的结构和性能进行了表征。通过在这些支架上培养人原代皮肤成纤维细胞（FEK4），利用SEM和MTS进行细胞黏附和增殖研究。实验结果表明，含有SS纳米颗粒的PCL/SS纳米纤维支架具有多孔非织造网状结构，纳米纤维相互交联。纤维直径非常均匀且精确，并且SS蛋白的二级结构未发生改变。此外，随着SS的添加，亲水性增强，这改善了FEK4细胞在支架上的黏附和增殖。