Li Zhifeng, Chen Zhong, Chang Ren'an
Department of Surgery, Affiliated Maternal and Child Care Service Centre of Nantong University, Nantong 226001, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2011 Apr;28(2):314-7.
This paper is aimed to observe the hepatocyte growth factor (HGF) loading and delivery ability of polylactic acid and oxygen carboxymethylated chitosan copolyer nanoparticles (PLA-O-CMC NPs). We prepared PLA-O-CMC NPs loaded with HGF by ultrasound in combination with magnetic stirring method. The NPs were characterized by transmission electron microscopy, embedding ratio; drug loading and drug delivery behaviors were observed by ELISA. The characteristics of PLA-O-CMC NPs loaded with HGF showed that the mean size was 139. 82 nm, polydispersity was 0.108, maximal HGF-embedding ratio was 76. 32%. The cumulative HGF release gradually increased in the first 24 hours in vitro, with sharp increasing in the first 7 hours, and moderate and steady increasing in the following 17 hours. The HGF had a burst release in the first 24 hours, and in this process the released HGF took up 36.7% of the whole release. From the second day,the HGF release decreased obviously, while it kept on releasing steadily (45-55 ng/d) for quite long time up to 30 days. The experiment proved that PLA-O-CMC NPs is a favourable carrier of HGF. PLA-O-CMC NPs loaded with HGF could rapidly release HGF in vitro. The released HGF reached the effective drug concentration and maintained the certain effective drug concentration for a long time.
本文旨在观察聚乳酸-氧羧甲基化壳聚糖共聚物纳米粒（PLA-O-CMC NPs）对肝细胞生长因子（HGF）的装载及递送能力。我们采用超声结合磁力搅拌法制备了负载HGF的PLA-O-CMC NPs。通过透射电子显微镜对纳米粒进行表征，测定包封率；采用酶联免疫吸附测定法观察载药及释药行为。负载HGF的PLA-O-CMC NPs的特性表明，其平均粒径为139.82 nm，多分散指数为0.108，最大HGF包封率为76.32%。体外实验中，HGF的累积释放量在最初24小时逐渐增加，前7小时急剧增加，随后17小时呈中度且稳定的增加。HGF在最初24小时有一个突释，在此过程中释放的HGF占总释放量的36.7%。从第二天起，HGF释放明显减少，但在长达30天的时间内持续稳定释放（45 - 55 ng/d）。实验证明PLA-O-CMC NPs是HGF的良好载体。负载HGF的PLA-O-CMC NPs在体外能快速释放HGF。释放的HGF能达到有效药物浓度并长时间维持一定的有效药物浓度。
