Liou G I, Geng L, al-Ubaidi M R, Matragoon S, Hanten G, Baehr W, Overbeek P A
Cullen Eye Institute, Baylor College of Medicine, Houston, Texas 77030.
J Biol Chem. 1990 May 25;265(15):8373-6.
Interphotoreceptor retinoid-binding protein (IRBP) is an extracellular protein that has been suggested to participate in the visual process as a carrier for visual retinoids. A chimeric gene composed of the human IRBP promoter fused to the bacterial reporter gene chloramphenicol acetyltransferase (CAT) was used to generate transgenic mice. Analysis of six transgenic families revealed that the CAT gene, concomitant with the endogenous IRBP gene, was expressed primarily in the retina and, to a lesser extent, in the pineal gland. These results establish that a 1.3-kilobase fragment from the 5' end of the human IRBP gene is sufficient to direct transgene expression to a visual subdivision of the central nervous system.
光感受器间类视黄醇结合蛋白（IRBP）是一种细胞外蛋白，有人认为它作为视觉类视黄醇的载体参与视觉过程。一个由人类IRBP启动子与细菌报告基因氯霉素乙酰转移酶（CAT）融合而成的嵌合基因被用于产生转基因小鼠。对六个转基因家系的分析表明，CAT基因与内源性IRBP基因一起，主要在视网膜中表达，在松果体中的表达程度较低。这些结果表明，来自人类IRBP基因5'端的一个1.3千碱基片段足以将转基因表达导向中枢神经系统的视觉分区。
