Hong Xiao-Lv, Cao Hong, Zhao Fang, Pan Xing-Fei, Zhang Ka, Xu Qi-Huan, Zhao Zhi-Xin, Li Gang
Department of Infectious Diseases, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2010 Dec;24(6):470-2.
To investigate the relationship between the SNP rs11614913 on miR196a-2 gene and the treatment effects of Peg-IFN-a plus Ribavirin on chronic hepatitis C patients.
The total 139 patients of chronic hepatitis C infection who received the treatment of Peg-IFN-alpha-2a or Peg-IFN-alpha-2b plus Ribavirin were enrolled in this study. The patients were divided into two groups: sustained virological response (SVR) (n = 82) group and non virological response (NVR) or recurrence (n = 57) group. Blood samples were collected and chromosomal DNA was extracted. The miR-196a-2 polymorphism was determined with the method of polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP).
In our study, there was statistically association between miR-196a-2 polymorphism and the antiviral therapy efficacy of hepatitis C patients. There was statistically significance in the CT genotype and the TT genotype of miR-196a-2 between the two groups [P = 0.009, A = 2.924 (1.285 -6.652)]. There was statistically significance in the CC genotype and the TT genotype between the two groups [P = 0.036, A = 3.091(1.052 -9.078)]. There was statistically significance in the C allele and the T allele between the two groups [P = 0.036, A = 3.091 (1.052 - 9.078)].
These findings suggested that the rs11614913 SNP in miR - 196a-2 be associated with the antiviral therapy efficacy of hepatitis C patients, and the TT genotype or T alleles be associated with the SVR while the CC genotype or C allele could be related to the NVR or recurrence.
探讨miR196a - 2基因上的单核苷酸多态性（SNP）rs11614913与聚乙二醇干扰素α（Peg - IFN - a）联合利巴韦林治疗慢性丙型肝炎患者疗效之间的关系。
选取139例接受Peg - IFN - α - 2a或Peg - IFN - α - 2b联合利巴韦林治疗的慢性丙型肝炎感染患者纳入本研究。将患者分为两组：病毒学持续应答（SVR）组（n = 82）和无病毒学应答（NVR）或复发组（n = 57）。采集血样并提取染色体DNA。采用聚合酶链反应（PCR）-限制性片段长度多态性（RFLP）方法检测miR - 196a - 2基因多态性。
在本研究中，miR - 196a - 2基因多态性与丙型肝炎患者抗病毒治疗疗效之间存在统计学关联。两组间miR - 196a - 2基因的CT基因型和TT基因型存在统计学差异[P = 0.009，比值比（OR）= 2.924（1.285 - 6.652）]。两组间CC基因型和TT基因型存在统计学差异[P = 0.036，OR = 3.091（1.052 - 9.078）]。两组间C等位基因和T等位基因存在统计学差异[P = 0.036，OR = 3.091（1.052 - 9.078）]。
这些研究结果表明，miR - 196a - 2基因中的rs11614913单核苷酸多态性与丙型肝炎患者抗病毒治疗疗效相关，TT基因型或T等位基因与病毒学持续应答相关，而CC基因型或C等位基因可能与无病毒学应答或复发相关。
