Merta A, Veselý J, Votruba I, Rosenberg I, Holý A
Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague.
Neoplasma. 1990;37(2):111-20.
Acyclic nucleotide analogs (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine (HPMPA) and 9-(2-phosphonylmethoxyethyl)adenine (PMEA) were phosphorylated in the presence of ribonucleoside 5'-triphosphates by the crude extract from mouse leukemia cells L1210 affording the respective mono- and diphosphoryl derivatives. The donor efficiency was decreasing in the order CTP greater than UTP greater than ATP greater than GTP. The presence of an ATP regenerating system stimulated considerably the conversion of both compounds. The rate of PMEA phosphorylation was 5-times slower than that of HPMPA both with and without an ATP regenerating system.
无环核苷酸类似物（S）-9-（3-羟基-2-膦酰甲氧基丙基）腺嘌呤（HPMPA）和9-（2-膦酰甲氧基乙基）腺嘌呤（PMEA）在核糖核苷5'-三磷酸存在下，被小鼠白血病细胞L1210的粗提物磷酸化，分别得到相应的单磷酸和二磷酸衍生物。供体效率按CTP＞UTP＞ATP＞GTP的顺序降低。ATP再生系统的存在显著刺激了这两种化合物的转化。无论有无ATP再生系统，PMEA的磷酸化速率都比HPMPA慢5倍。
