Yanez A, Sabbe M B, Stevens C W, Yaksh T L
Departamento de Anesthesia, H. Del Insalud A. Marcide, Carretera de Catabois, Ferrol, Spain.
Neuropharmacology. 1990 Apr;29(4):359-64. doi: 10.1016/0028-3908(90)90094-8.
The antinociceptive properties, as measured by the tail-flick and hot-plate tests, and the motor effects of an intrathecally-administered benzodiazepine agonist midazolam, alone, and in combination with morphine, was examined in rats. Midazolam alone produced a weak but dose-dependent (20-60 micrograms) antinociceptive effect in addition to a clear motor dysfunction at larger doses (60-100 micrograms). An inactive dose of intrathecally-administered midazolam (20 micrograms) produced a leftward shift in the dose-response curve for intrathecally administered morphine, in the thermal antinociceptive tests. This supra-additive effect was antagonized by naloxone (1 mg/kg). The data suggest a synergistic interaction between mu- and GABAA-receptors in the spinal processing of thermally-evoked pain.
通过甩尾试验和热板试验测量，对鞘内注射苯二氮䓬激动剂咪达唑仑单独以及与吗啡联合使用时在大鼠身上的镇痛特性和运动效应进行了研究。单独使用咪达唑仑除了在较大剂量（60 - 100微克）时会产生明显的运动功能障碍外，还会产生微弱但剂量依赖性（20 - 60微克）的镇痛作用。在热镇痛试验中，鞘内注射无活性剂量的咪达唑仑（20微克）会使鞘内注射吗啡的剂量 - 反应曲线向左移动。这种超相加效应被纳洛酮（1毫克/千克）拮抗。数据表明在热诱发疼痛的脊髓处理过程中，μ受体和GABAA受体之间存在协同相互作用。
