Institute of Neurophysiology, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Neuroendocrinology. 2011;94(3):191-9. doi: 10.1159/000328128. Epub 2011 May 21.
The amygdala receives afferent sensory input and processes information related to hydromineral balance. Angiotensin acts on and through the amygdala to stimulate thirst and sodium appetite. In addition, different angiotensins seem to play a role in cognition and learning mechanisms by acting on and through the amygdala. Recently, we showed that angiotensin-(1-7) (Ang-(1-7)) enhances the magnitude of long-term potentiation (LTP) in the lateral nucleus of the amygdala (LA) via the Mas receptor.
Extracellular field potentials were measured in the LA.
LA-LTP induced by stimulation of the external capsule was nitric oxide (NO)-dependent because the NO synthase (NOS) inhibitor L-NAME reduced LA-LTP. The LA-LTP was also reduced in both male and female nNOS and eNOS knockout mice. In male eNOS(-/-) mice, Ang-(1-7) enhanced LA-LTP, whereas the LTP-enhancing effect of Ang-(1-7) was missing in female eNOS(-/-) mice. Therefore, the LTP-enhancing effect of Ang-(1-7) was mediated by eNOS in females. In contrast, Ang-(1-7) strongly enhanced the LTP in nNOS(-/-) females, whereas the effect of Ang-(1-7) was missing in nNOS(-/-) males. Thus, Ang-(1-7) induced an increase in the magnitude of LTP via the involvement of nNOS in males.
Our data support not only the hypothesis that NO contributes to plasticity changes in the lateral amygdala, but also show for the first time a gender-dependent involvement of different isoforms of NOS in the mediation of Ang-(1-7) on LTP in the amygdala.
杏仁核接收传入的感觉输入，并处理与水盐平衡相关的信息。血管紧张素通过杏仁核作用并刺激口渴和钠食欲。此外，不同的血管紧张素似乎通过作用于杏仁核来参与认知和学习机制。最近，我们发现血管紧张素-(1-7)（Ang-(1-7)）通过 Mas 受体增强外侧杏仁核（LA）中的长时程增强（LTP）的幅度。
在 LA 中测量细胞外场电位。
刺激外囊引起的 LA-LTP 依赖于一氧化氮（NO），因为 NO 合酶（NOS）抑制剂 L-NAME 降低了 LA-LTP。LA-LTP 在雄性和雌性 nNOS 和 eNOS 敲除小鼠中也降低。在雄性 eNOS(-/-) 小鼠中，Ang-(1-7) 增强了 LA-LTP，而 Ang-(1-7) 的 LTP 增强作用在雌性 eNOS(-/-) 小鼠中缺失。因此，Ang-(1-7) 的 LTP 增强作用在雌性中通过 eNOS 介导。相比之下，Ang-(1-7) 在 nNOS(-/-) 雌性中强烈增强 LTP，而 Ang-(1-7) 的作用在 nNOS(-/-) 雄性中缺失。因此，Ang-(1-7) 通过涉及 nNOS 在雄性中诱导 LTP 幅度增加。
我们的数据不仅支持 NO 有助于外侧杏仁核可塑性变化的假设，而且首次表明不同 NOS 同工型在 Ang-(1-7) 对杏仁核 LTP 的介导中存在性别依赖性。
