Williams C J, Major P P, Dion A S
Institute for Molecular Genetics, Center for Molecular Medicine and Immunology, Newark, N.J.
Tumour Biol. 1990;11(3):145-57. doi: 10.1159/000217649.
The mouse monoclonal antibody MA5, generated versus a membrane-enriched extract of breast cancer metastatic to liver, detects one or two high molecular weight species (greater than 200 kD) in breast tumor membranes, human milk fat globule membranes, and various breast tumor cell lines. From comparative studies of five breast carcinoma lines (BT20, BT549, MCF-7, T47D, and ZR75-1), as well as an epithelial line established from milk (HBL-100), we report the stimulation of expression of MA5-reactive antigen in a mucinous breast tumor cell line (BT549) through the use of a culture medium supplemented with charcoal-absorbed fetal calf serum, insulin, and hydrocortisone. Large amounts of aggregated MA5-reactive antigen are secreted into the culture medium and can be recovered from the media for further purification by centrifugation. These findings suggest that BT549 cells, grown in the special nutritive medium, may be useful in providing an ample source of epithelial membrane antigen (also termed polymorphic epithelial mucin) for standardization of clinical assay protocols, as well as provide a model system for studies of the regulation of expression for this class of antigens in breast carcinoma.
小鼠单克隆抗体MA5是针对转移至肝脏的乳腺癌膜富集提取物产生的，它能在乳腺肿瘤膜、人乳脂肪球膜及多种乳腺肿瘤细胞系中检测到一种或两种高分子量物质（大于200 kD）。通过对五种乳腺癌细胞系（BT20、BT549、MCF-7、T47D和ZR75-1）以及一种从乳汁建立的上皮细胞系（HBL-100）进行比较研究，我们报告了在一种黏液性乳腺肿瘤细胞系（BT549）中，通过使用添加了经活性炭吸附的胎牛血清、胰岛素和氢化可的松的培养基，MA5反应性抗原的表达受到刺激。大量聚集的MA5反应性抗原分泌到培养基中，可通过离心从培养基中回收以进行进一步纯化。这些发现表明，在特殊营养培养基中生长的BT549细胞，可能有助于为临床检测方案的标准化提供丰富的上皮膜抗原（也称为多形性上皮黏蛋白）来源，以及为研究这类抗原在乳腺癌中的表达调控提供一个模型系统。
