Ergazaki M, Xinarianos G, Giannoudis A, Koffa M, Liloglou T, Kattamis C, Spandidos D
NATL HELLEN RES FND,INST BIOL RES & BIOTECHNOL,GR-11635 ATHENS,GREECE. UNIV CRETE,SCH MED,CLIN VIROL LAB,IRAKLION,GREECE. UNIV ATHENS,ST SOPHIA CHILDRENS HOSP,SCH MED,DEPT PEDIAT 1,GR-11527 ATHENS,GREECE.
Oncol Rep. 1994 Jul;1(4):813-6. doi: 10.3892/or.1.4.813.
Infections caused by Human Cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) are common in multiple transfused patients, such as patients with beta-thalassaemia. The ability of the Polymerase Chain Reaction (PCR) to amplify HCMV and EBV DNA from blood and other samples makes this technique a valuable diagnostic tool for the detection of both viruses in the early stages of the infection. PCR was used for the amplification of a 435 bp region of the immediate early-1 (IE-1) gene of HCMV and a 375 bp sequence from the EcoRI B fragment of EBV genome. Blood samples from 80 patients with beta-thalassaemia were examined. HCMV was found in 14 and EBV in 12 patients. The results obtained confirm the implications of HCMV and EBV in the diagnosis of viral infections in multiple transfused patients as well as the importance of PCR technique as a valuable diagnostic tool.
人巨细胞病毒（HCMV）和爱泼斯坦-巴尔病毒（EBV）引起的感染在多次输血的患者中很常见，如β地中海贫血患者。聚合酶链反应（PCR）能够从血液和其他样本中扩增HCMV和EBV DNA，使得该技术成为在感染早期检测这两种病毒的有价值的诊断工具。PCR用于扩增HCMV即刻早期-1（IE-1）基因的435 bp区域以及EBV基因组EcoRI B片段的375 bp序列。对80例β地中海贫血患者的血液样本进行了检测。14例患者检测到HCMV，12例患者检测到EBV。所得结果证实了HCMV和EBV在多次输血患者病毒感染诊断中的意义，以及PCR技术作为有价值诊断工具的重要性。
