[The effect of dibutyryl derivatives of cyclic nucleotides on albumin production in a primary culture of rat liver cells].

Dibutyryl-derivative of cyclic AMP (Bt2cAMP, 10(-3)M) affected the basal and cortisol-induced production of immunoreactive serum albumin (SA) in primary cultures of fetal rat liver cells by the type of two-phase time dependency. The same two-phase character exhibited dose-dependent effect of Bt2cAMP (10(-5)-10(-3)M) on SA production in cultured liver cells obtained from fetal and 3 weeks old rats. Similar two-phase dose- and time-dependent effects demonstrated Bt2cGMP (10(-5)-10(-3)M), although certain differences were detected when either action of Bt2cAMP and Bt2cGMP or response of cultured liver cells from fetal and early postnatal rats to these drugs were studied. The data obtained suggest that normal liver cells are provided with feed-back mechanism which limited the cellular reaction to cyclic nucleotides under experimental conditions involving, potentially excessive doses and/or time of action of these agents.