Poncet D, Corthier G, Charpilienne A, Cohen J
Station de Virologie et d'Immunologie Moléculaires INRA, C.R.J. Domaine de Vilvert, Jouy-en-Josas, France.
Virus Res. 1990 Mar;15(3):267-74. doi: 10.1016/0168-1702(90)90034-9.
cDNA molecules encoding the major structural protein (VP6) of the Simian rotavirus SA11 were inserted under the control of the vaccinia virus 7.5 kDa promoter into the thymidine kinase gene. Synthesis of VP6 was demonstrated by immunoprecipitation of recombinant virus-infected cell. Mice inoculated via several routes with this recombinant vaccinia produce high titers of antirotavirus antibodies lacking neutralizing activity.
编码猿猴轮状病毒SA11主要结构蛋白（VP6）的cDNA分子，在痘苗病毒7.5kDa启动子的控制下，被插入到胸苷激酶基因中。通过对重组病毒感染细胞进行免疫沉淀，证实了VP6的合成。通过多种途径接种这种重组痘苗病毒的小鼠，产生了高滴度但缺乏中和活性的抗轮状病毒抗体。
