Entomology and Nematology Department, Citrus Research and Education Center, University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USA.
Microb Ecol. 2011 Oct;62(3):690-703. doi: 10.1007/s00248-011-9872-1. Epub 2011 May 24.
Container-breeding mosquitoes, such as Aedes triseriatus, ingest biofilms and filter water column microorganisms directly to obtain the bulk of their nutrition. Scirtid beetles often co-occur with A. triseriatus and may facilitate the production of mosquito adults under low-resource conditions. Using molecular genetic techniques and quantitative assays, we observed changes in the dynamics and composition of bacterial and fungal communities present on leaf detritus and in the water column when scirtid beetles co-occur with A. triseriatus. Data from terminal restriction fragment polymorphism analysis indicated scirtid presence alters the structure of fungal communities in the water column but not leaf-associated fungal communities. Similar changes in leaf and water bacterial communities occurred in response to mosquito presence. In addition, we observed increased processing of leaf detritus, higher leaf-associated enzyme activity, higher bacterial productivity, and higher leaf-associated fungal biomass when scirtid beetles were present. Such shifts suggest beetle feeding facilitates mosquito production indirectly through the microbial community rather than directly through an increase in available fine particulate organic matter.
容器滋生的蚊子，如三带喙库蚊，直接摄入生物膜并过滤水柱中的微生物来获取大部分营养。豉甲常与三带喙库蚊共同出现，并且可能在资源匮乏的条件下促进蚊子成虫的产生。利用分子遗传技术和定量检测方法，我们观察到当豉甲与三带喙库蚊共同存在时，叶凋落物和水柱中细菌和真菌群落的动态和组成发生了变化。末端限制性片段多态性分析的数据表明，豉甲的存在改变了水柱中真菌群落的结构，但不改变与叶相关的真菌群落。蚊子的存在也导致了叶和水细菌群落的相似变化。此外，当豉甲存在时，我们观察到叶凋落物的处理量增加，叶相关酶活性更高，细菌生产力更高，叶相关真菌生物量更高。这些变化表明，甲虫的摄食通过微生物群落而不是通过增加可用的细颗粒有机物质间接促进蚊子的产生。
