Catalase activity was partially purified from body wall muscle of the parasitic nematode, Ascaris suum, and was similar to catalases isolated from mammalian tissues. It exhibited a broad pH optimum and was unaffected by 2 mM ethylenediaminetetra-acetate. In contrast, it was inhibited reversibly by 1 mM cyanide and irreversibly by prior incubation in 40 mM 3-amino-1:2:4-triazole for 1 hr or heating at 80 degrees C for 15 min. 2. Catalase activity was highest in the unembryonated "egg" and decreased dramatically as development proceeded. 3. Catalase activity in adult body wall muscle was similar to that in rat skeletal muscle, but dramatically lower than that in rat liver. Catalase activity was barely detectable in A. suum testis. 4. Cytochrome-c peroxidase activity did not appear to be present in adult A. suum muscle mitochondria.
摘要
过氧化氢酶活性是从寄生线虫猪蛔虫的体壁肌肉中部分纯化得到的，它与从哺乳动物组织中分离出的过氧化氢酶相似。它表现出较宽的最适pH值，且不受2 mM乙二胺四乙酸的影响。相比之下，它可被1 mM氰化物可逆抑制，在40 mM 3-氨基-1:2:4-三唑中预先孵育1小时或在80℃加热15分钟则会被不可逆抑制。2. 过氧化氢酶活性在未受精的“卵”中最高，并随着发育进程急剧下降。3. 成年体壁肌肉中的过氧化氢酶活性与大鼠骨骼肌中的相似，但远低于大鼠肝脏中的。在猪蛔虫睾丸中几乎检测不到过氧化氢酶活性。4. 细胞色素c过氧化物酶活性在成年猪蛔虫肌肉线粒体中似乎不存在。
