Laboratory of Molecular Genetics, Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 70101, Taiwan.
Fish Shellfish Immunol. 2011 Aug;31(2):232-42. doi: 10.1016/j.fsi.2011.05.008. Epub 2011 May 17.
Mammalian secreted protein acidic and rich in cysteine (SPARC) is the primary regulator of cell shape and cell adhesion to fibronectin. We, for the first time, report the complete sequencing of SPARC cDNA from orange-spotted grouper. Despite the difference in the lengths of the SPARC transcripts, all of the SPARC molecules encoded a signal peptide, follistain-like copper binding sequence (KGHK) domain, and extracellular domain. The grouper SPARC gene was differentially expressed in vivo and contributed differently to high-level expression of SPARC in muscle. Immunohistochemical staining demonstrated a decreased level of SPARC in nodavirus-infected grouper compared with healthy grouper. Comparative real-time polymerase chain reaction analyses of eye tissues of viral nervous necrosis grouper and healthy grouper were performed. Recombinant SPARC produced changes in grouper cell shape 24 h after treatment. The results provide new insight into the pathogenesis of nodavirus, and demonstrate an experimental rationale for SPARC characterization in nodavirus-infected grouper.
哺乳动物分泌的富含半胱氨酸的酸性蛋白（SPARC）是调节细胞形状和细胞与纤维连接蛋白黏附的主要调控因子。我们首次报道了来自橘点石斑鱼的 SPARC cDNA 的完整测序。尽管 SPARC 转录本的长度存在差异，但所有的 SPARC 分子都编码了一个信号肽、卵泡抑素样铜结合序列（KGHK）域和细胞外结构域。在体内，石斑鱼的 SPARC 基因表达存在差异，并且对肌肉中 SPARC 的高水平表达有不同的贡献。免疫组织化学染色显示，与健康石斑鱼相比，感染神经坏死病毒的石斑鱼 SPARC 水平降低。对神经坏死病毒感染的石斑鱼和健康石斑鱼的眼部组织进行了比较实时聚合酶链反应分析。重组 SPARC 处理 24 小时后改变了石斑鱼细胞的形状。这些结果为神经坏死病毒的发病机制提供了新的见解，并为神经坏死病毒感染的石斑鱼中 SPARC 特征的实验原理提供了依据。
