Faculty of Education and Human Sciences, University of Yamanashi, Kofu, Yamanashi 400-8510, Japan.
Mol Phylogenet Evol. 2011 Sep;60(3):416-27. doi: 10.1016/j.ympev.2011.05.005. Epub 2011 May 17.
The evolutionary history of eight-barbel loaches of the genus Lefua contains important phylogenetic information that will aid in resolution of the faunal formations and evolutionary histories of Japanese and East Asian freshwater fishes. Our sequencing of the mitochondrial D-loop region in a large number of samples allowed construction of the most comprehensive phylogeny of these loaches to date; we demonstrated monophyly of five Lefua species and identified populations of Lufua. sp. and Lefua echigonia. Loaches inhabiting the Tokai region in Japan were morphologically and ecologically indistinguishable from Lefua sp. However, they were included in the L. echigonia lineage. We determined a novel phylogeny by sequencing the nuclear ribosomal S7 subunit and showed that nuclear DNA phylogeny essentially matched the mitochondrial DNA phylogeny. Loaches from the Tokai region were part of the L. echigonia lineage, indicating parallel evolution between Tokai loaches and Lefua sp. in western Japan. We presented the most robust phylogeny to date using concatenated mitochondrial and nuclear sequences. The wealth of molecular information allowed us to speculate on evolutionary processes in the genus Lefua.
八须鲶属 Lefua 的进化历史包含了重要的系统发育信息，有助于解决日本和东亚淡水鱼类的区系形成和进化历史。我们对大量样本的线粒体 D 环区域进行了测序，构建了迄今为止最全面的八须鲶属系统发育关系；我们证明了五个 Lefua 物种的单系性，并鉴定了 Lufua.sp.和 Lefua echigonia. 的种群。日本东海地区的鲶鱼在形态和生态上与 Lefua.sp.无法区分。然而，它们被归入了 L. echigonia 谱系。我们通过测序核核糖体 S7 亚基确定了一个新的系统发育关系，并表明核 DNA 系统发育与线粒体 DNA 系统发育基本一致。东海地区的鲶鱼属于 L. echigonia 谱系，表明东海鲶鱼和日本西部的 Lefua.sp.之间存在平行进化。我们使用串联的线粒体和核序列呈现了迄今为止最稳健的系统发育关系。丰富的分子信息使我们能够推测 Lefua 属的进化过程。
