Kettler A H, Rutledge M, Tschen J A, Buffone G
Department of Dermatology, Baylor College of Medicine, Houston, Tex. 77030.
Arch Dermatol. 1990 Jun;126(6):777-81.
Genital Bowen's disease has been strongly linked in recent studies to human papillomavirus (HPV). Nongenital Bowen's disease has been less well investigated, although isolated reports, all of which involved detection of HPV after extraction of DNA from fresh-frozen tissue, have been made. We investigated 25 cases of nongenital Bowen's disease in 5 black and 20 white patients for the presence of HPV types 1, 6, 11, 16, and 18 using paraffin-embedded tissues. Human papillomavirus was present in six specimens from 3 of the 5 black patients (one previously reported to be positive on Southern blot) and 3 of the 20 white patients; HPV 16 was detected in all 6 cases on low-stringency testing, but only 4 remained positive on high-stringency testing. This suggests an HPV closely related to but not entirely homologous with HPV 16 in the 2 remaining cases. Five of the 6 positive specimens were lesions from the hands and feet and 1 was from the volar aspect of the arm. Clinical factors associated with the presence of HPV included black race, location on the palmar surface and the feet, young age, and verrucous or hyperkeratotic clinical appearance. Of the 6 positive cases, all 5 of the patients available for examination also had evidence of HPV-associated genital lesions. No specific histopathologic features were found to be indicative of the presence or absence of HPV.
近期研究表明，生殖器鲍温病与人类乳头瘤病毒（HPV）密切相关。非生殖器鲍温病的研究较少，尽管有个别报告，所有这些报告均涉及从新鲜冷冻组织中提取DNA后检测HPV。我们使用石蜡包埋组织，对5名黑人患者和20名白人患者的25例非生殖器鲍温病病例进行了HPV 1、6、11、16和18型的检测。在5名黑人患者中的3例（其中1例先前经Southern印迹法检测为阳性）和20名白人患者中的3例的6个标本中检测到HPV；在低严谨度检测中，所有6例均检测到HPV 16，但在高严谨度检测中只有4例仍为阳性。这表明在其余2例中存在一种与HPV 16密切相关但并非完全同源的HPV。6个阳性标本中有5个是手足部病变，1个是手臂掌侧病变。与HPV存在相关的临床因素包括黑人种族、手掌和足部位置、年轻以及疣状或角化过度的临床表现。在6例阳性病例中，所有5例可供检查的患者也有HPV相关生殖器病变的证据。未发现特定的组织病理学特征可表明HPV的存在与否。
