State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, People's Republic of China.
Nanotechnology. 2011 Jul 8;22(27):275715. doi: 10.1088/0957-4484/22/27/275715. Epub 2011 May 25.
The localized plasmon controlled fluorescence has been discussed by comparing the fluorescence enhancement of dyes on different shaped silver nanoparticle self-assembled films. A trilayer structure, composed of a silver nanoparticle monolayer, a proper thickness polyelectrolyte spacing layer and a dye-adsorbed layer, was constructed to study the plasmon enhanced fluorescence properties. The effective coupling of the plasmon band with the excitation or emission of dye resulted in different enhancement factors. Moreover, the plasmon enhanced fluorescence resonance energy transfer (FRET) of two dyes was observed. The FRET efficiency of the spherical silver nanoparticle self-assembled film had a 2.8-fold increase. The improvement of FRET efficiency via localized surface plasmons would increase the sensitivity of FRET-based bioassays.
通过比较不同形状的银纳米粒子自组装膜上染料的荧光增强，讨论了局域等离子体控制的荧光。构建了由银纳米粒子单层、适当厚度的聚电解质间隔层和染料吸附层组成的三层结构，以研究等离子体增强荧光特性。等离子体带与染料的激发或发射的有效耦合导致不同的增强因子。此外，还观察到两种染料的等离子体增强荧光共振能量转移（FRET）。球形银纳米粒子自组装膜的 FRET 效率增加了 2.8 倍。通过局域表面等离激元提高 FRET 效率将提高基于 FRET 的生物分析的灵敏度。
